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	Production of mice using ips cells and tetraploid complementation
	Zhao, Xiao-Yang1,4; Lv, Zhuo1,4; Li, Wei1,4; Zeng, Fanyi2,3; Zhou, Qi1
刊名	Nature protocols
	2010
卷号	5期号:5页码:963-971
ISSN号	1754-2189
DOI	10.1038/nprot.2010.61
通讯作者	Zhou, qi(qzhou@ioz.ac.cn)
英文摘要	Induced pluripotent stem cells (ipscs) are considered to be an attractive alternative to embryonic stem cells (escs) and may provide great potential for clinical applications in regenerative medicine. although possessing characteristics similar to escs, the true pluripotency of these newly studied ipscs was not known because none of the previously developed ipscs passed the tetraploid complementation assay, which is regarded as the most stringent test for pluripotency. we have recently shown that by modifying some of the culture conditions for inducing ipscs, we were able to generate cell lines of high pluripotency, resulting in the production of live-born, fertile animals through tetraploid complementation. in this paper, we describe details of our methods of generating ips cell lines and subsequently producing full-term live animals through the tetraploid complementation assay; the procedure can be completed within 2 months.
WOS关键词	PLURIPOTENT STEM-CELLS ; GENERATION ; EMBRYOS
WOS研究方向	Biochemistry & Molecular Biology
WOS类目	Biochemical Research Methods
语种	英语
出版者	NATURE PUBLISHING GROUP
WOS记录号	WOS:000277174100015
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2413083
专题	中国科学院大学
通讯作者	Zhou, Qi
作者单位	1.Chinese Acad Sci, Inst Zool, State Key Lab Reprod Biol, Beijing, Peoples R China 2.Shanghai Jiao Tong Univ, Inst Med Genet, Shanghai 200030, Peoples R China 3.Shanghai Jiao Tong Univ, Inst Med Sci, Shanghai 200030, Peoples R China 4.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Zhao, Xiao-Yang,Lv, Zhuo,Li, Wei,et al. Production of mice using ips cells and tetraploid complementation[J]. Nature protocols,2010,5(5):963-971.
APA	Zhao, Xiao-Yang,Lv, Zhuo,Li, Wei,Zeng, Fanyi,&Zhou, Qi.(2010).Production of mice using ips cells and tetraploid complementation.Nature protocols,5(5),963-971.
MLA	Zhao, Xiao-Yang,et al."Production of mice using ips cells and tetraploid complementation".Nature protocols 5.5(2010):963-971.

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