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	Molecular bases of thioredoxin and thioredoxin reductase-mediated prooxidant actions of (-)-epigallocatechin-3-gallate
	Zhang, Huihui1; Cao, Dong2,3; Cui, Wei4; Ji, Mingjuan4; Qian, Xiaohong2; Zhong, Liangwei1
刊名	Free radical biology and medicine
	2010-12-15
卷号	49期号:12页码:2010-2018
关键词	Thioredoxin Thioredoxin reductase (-) epigallocatechin 3 gallate Egcg Ros Auto oxidation
ISSN号	0891-5849
DOI	10.1016/j.freeradbiomed.2010.09.031
通讯作者	Zhong, liangwei()
英文摘要	Thioredoxin (trx) and thioredoxin reductase (trxr) function as antioxidant and anti-apoptotic proteins which are often up-regulated in drug-resistant cancer cells (-)-epigallocatechin-3-gallate (egcg) is a naturally occurring antioxidant in green tea but also exhibits prooxidant and apoptosis-inducing properties we have previously showed a linkage between egcg-induced inactivation of trxr and decreased cell survival revealing trxr as a new target of egcg however the molecular events underlying the importance of trx/trxr in egcg-induced cytotoxicity remain unclear here we show that the crosstalk between egcg and trx/trxr occurred in a redox-dependent manner and egcg induced inactivation of trx/trxr in parallel with increased ros levels in hela cells moreover egcg displayed great reactivity with cys/sec residues that have low pk(a) values the structure of egcg suggests that its quinone form would readily react with thiolate and selenolate nucleophiles using mass spectrometry we have demonstrated the formation of egcg trx1 (cys(32)) and egcg-trxr (cys/sec) conjugates confirming that egcg quinone specifically conjugates with active-site cys(32) in trx or c-terminal cys/selenocysteine (sec) couple in trxr under conditions where trx/trxr are reduced non-reduced form of trx/trxr could escape from egcg inhibition these data reveal a potential mechanism for enhancing egcg-induced cancer cell death by the nadph-dependent reduction of trx/trxr (c) 2010 elsevier inc all rights reserved
WOS关键词	CIS-DIAMMINEDICHLOROPLATINUM II ; GREEN TEA POLYPHENOLS ; MAMMALIAN THIOREDOXIN ; MASS-SPECTROMETRY ; GLUTATHIONE-REDUCTASE ; IN-VITRO ; ANTIOXIDANT ; CELL ; EPIGALLOCATECHIN-3-GALLATE ; CATECHINS
WOS研究方向	Biochemistry & Molecular Biology ; Endocrinology & Metabolism
WOS类目	Biochemistry & Molecular Biology ; Endocrinology & Metabolism
语种	英语
出版者	ELSEVIER SCIENCE INC
WOS记录号	WOS:000285445000018
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2412291
专题	中国科学院大学
通讯作者	Zhong, Liangwei
作者单位	1.Chinese Acad Sci, Coll Life Sci, Grad Univ, Beijing 100049, Peoples R China 2.Beijing Proteome Res Ctr, State Key Lab Prote, Beijing Inst Radiat Med, Beijing 102206, Peoples R China 3.Chinese Acad Sci, State Key Lab Environm Chem & Ecotoxicol, Res Ctr Eco Environm Sci, Beijing 100085, Peoples R China 4.Chinese Acad Sci, Coll Chem & Chem Engn, Grad Univ, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Huihui,Cao, Dong,Cui, Wei,et al. Molecular bases of thioredoxin and thioredoxin reductase-mediated prooxidant actions of (-)-epigallocatechin-3-gallate[J]. Free radical biology and medicine,2010,49(12):2010-2018.
APA	Zhang, Huihui,Cao, Dong,Cui, Wei,Ji, Mingjuan,Qian, Xiaohong,&Zhong, Liangwei.(2010).Molecular bases of thioredoxin and thioredoxin reductase-mediated prooxidant actions of (-)-epigallocatechin-3-gallate.Free radical biology and medicine,49(12),2010-2018.
MLA	Zhang, Huihui,et al."Molecular bases of thioredoxin and thioredoxin reductase-mediated prooxidant actions of (-)-epigallocatechin-3-gallate".Free radical biology and medicine 49.12(2010):2010-2018.

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