| Two electrophoreses in different ph buffers to purify forest soil dna contaminated with humic substances | |
| Hou, Weiguo1,2; Lian, Bin1; Rothenberg, Sarah1 | |
| 刊名 | African journal of biotechnology
 |
| 2010-04-19 | |
| 卷号 | 9期号:16页码:2401-2407 |
| 关键词 | Dna extraction Forest soil Humic substances Ph Specific charge Two-step electrophoreses |
| ISSN号 | 1684-5315 |
| 通讯作者 | Lian, bin(bin2368@vip.163.com) |
| 英文摘要 | Direct extraction of dna from soils is a useful way to gain genetic information on the soil source. however, dna extraction from soils, especially forest soils, may be contaminated by humic substances due to their similar physical and chemical characteristics to soil. even commercial soil dna extraction kits fail to retrieve dna from these soils. using the potential changes of specific charge of dna and humic substances in a ph solution, we performed two electrophoreses in different ph buffers to eliminate the interfering substances. the method produced high quality soil dna, which is applicable for pcr amplification. |
| WOS关键词 | POLYMERASE-CHAIN-REACTION ; ENVIRONMENTAL-SAMPLES ; NIFH GENE ; BACTERIAL COMMUNITY ; EXTRACTION ; AMPLIFICATION ; PURIFICATION ; DIVERSITY ; SEDIMENTS ; PCR |
| WOS研究方向 | Biotechnology & Applied Microbiology |
| WOS类目 | Biotechnology & Applied Microbiology |
| 语种 | 英语 |
| 出版者 | ACADEMIC JOURNALS |
| WOS记录号 | WOS:000277015700011 |
| 内容类型 | 期刊论文 |
| URI标识 | http://www.corc.org.cn/handle/1471x/2409770 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Lian, Bin |
| 作者单位 | 1.Chinese Acad Sci, Inst Geochem, Key State Lab Environm Geochem, Guiyang, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Hou, Weiguo,Lian, Bin,Rothenberg, Sarah. Two electrophoreses in different ph buffers to purify forest soil dna contaminated with humic substances[J]. African journal of biotechnology,2010,9(16):2401-2407. |
| APA | Hou, Weiguo,Lian, Bin,&Rothenberg, Sarah.(2010).Two electrophoreses in different ph buffers to purify forest soil dna contaminated with humic substances.African journal of biotechnology,9(16),2401-2407. |
| MLA | Hou, Weiguo,et al."Two electrophoreses in different ph buffers to purify forest soil dna contaminated with humic substances".African journal of biotechnology 9.16(2010):2401-2407. |
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