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	Opposing regulation of histamine-induced calcium signaling by sodium selenite and ebselen via alterations of thiol redox status
	Zhang, Huihui; Zhong, Liangwei
刊名	European journal of pharmacology
	2010-01-25
卷号	626期号:2-3页码:276-282
关键词	Calcium signaling Ebselen Histamine Sodium selenite Thioredoxin reductase
ISSN号	0014-2999
DOI	10.1016/j.ejphar.2009.09.034
通讯作者	Zhong, liangwei(liazho@gucas.ac.cn)
英文摘要	Elevated blood histamine plays a role in the pathogenesis of atherosclerosis. calcium signaling mediates histamine action in endothelial cells. selenium (se) is a dietary essential trace element for humans. se compounds in different oxidation states were found to exhibit an opposing effect on the histamine-induced calcium signaling in the ecv304 cell line. when se in the form of sodium selenite was added in the cell culture, the reactivity of the histamine hi-receptor was increased as reported in our previous paper. we here show that as a culture supplement, sodium selenite enhanced the activity of selenoprotein thioredoxin reductase (trxr) and the calcium response to histamine stimulation, which were reversed by treating the cells with gold thioglucose, a nucleophilic drug that selectively modifies thiolate/selenolate groups. sodium selenite most likely caused a reductive shift in the thiol/disulfide redox balance through increasing trxr activity. in contrast, when the cells were treated with se in the form of ebselen, a thiol oxidant with peroxidase-like activity, histamine-induced calcium release and calcium entry were significantly suppressed. this effect appeared related to the thiol-directed modification rather than the peroxidase-like activity of ebselen, because this inhibitory effect was not replicated by increasing cellular peroxidase activity. thus, the opposing effects of sodium selenite and ebselen on histamine-induced calcium signaling are achieved, at least in part, through their opposite actions in modulating the thiol/disulfide redox state. (c) 2009 elsevier b.v. all rights reserved.
WOS关键词	ATTENUATES OXIDATIVE STRESS ; HUMAN ENDOTHELIAL-CELLS ; CORONARY-HEART-DISEASE ; NITRIC-OXIDE SYNTHASE ; PROTEIN-KINASE-C ; THIOREDOXIN REDUCTASE ; SMOOTH-MUSCLE ; PHOSPHOLIPASE-C ; P-SELECTIN ; INHIBITION
WOS研究方向	Pharmacology & Pharmacy
WOS类目	Pharmacology & Pharmacy
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000274090800026
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2408869
专题	中国科学院大学
通讯作者	Zhong, Liangwei
作者单位	Chinese Acad Sci, Grad Univ, Coll Life Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Huihui,Zhong, Liangwei. Opposing regulation of histamine-induced calcium signaling by sodium selenite and ebselen via alterations of thiol redox status[J]. European journal of pharmacology,2010,626(2-3):276-282.
APA	Zhang, Huihui,&Zhong, Liangwei.(2010).Opposing regulation of histamine-induced calcium signaling by sodium selenite and ebselen via alterations of thiol redox status.European journal of pharmacology,626(2-3),276-282.
MLA	Zhang, Huihui,et al."Opposing regulation of histamine-induced calcium signaling by sodium selenite and ebselen via alterations of thiol redox status".European journal of pharmacology 626.2-3(2010):276-282.

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