Fast isolation of highly active photosystem ii core complexes from spinach | |
Wang, Zhao-Gai1,2,3; Xu, Tian-Hua1,2; Liu, Cheng1; Yang, Chun-Hong1 | |
刊名 | Journal of integrative plant biology
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2010-09-01 | |
卷号 | 52期号:9页码:793-800 |
ISSN号 | 1672-9072 |
DOI | 10.1111/j.1744-7909.2010.00971.x |
通讯作者 | Yang, chun-hong(yangch@ibcas.ac.cn) |
英文摘要 | Purification of photosystem ii (psii) core complexes is a time-consuming and low-efficiency process. in order to isolate pure and active psii core complexes in large amounts, we have developed a fast method to isolate highly active monomeric and dimeric psii core complexes from spinach leaves by using sucrose gradient ultracentrifugation. by using a vertical rotor the process was completed significantly faster compared with a swing-out rotor. in order to keep the core complexes in high activity, the whole isolation procedure was performed in the presence of glycine betain and ph at 6.3. the isolated pigment-protein complexes were characterized by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, absorption spectroscopy, 77 k fluorescence spectroscopy and high performance liquid chromatography. our results show that this method is a better choice for quick and efficient isolation of functionally active psii core complexes. |
WOS关键词 | THYLAKOID MEMBRANES ; EXTRINSIC PROTEINS ; DENSITY GRADIENT ; VERTICAL ROTOR ; BINDING ; CHLAMYDOMONAS ; PURIFICATION ; FLUORESCENCE ; PARTICLES ; DETERGENT |
WOS研究方向 | Biochemistry & Molecular Biology ; Plant Sciences |
WOS类目 | Biochemistry & Molecular Biology ; Plant Sciences |
语种 | 英语 |
出版者 | WILEY-BLACKWELL |
WOS记录号 | WOS:000281216900003 |
内容类型 | 期刊论文 |
URI标识 | http://www.corc.org.cn/handle/1471x/2408422 |
专题 | 中国科学院大学 |
通讯作者 | Yang, Chun-Hong |
作者单位 | 1.Chinese Acad Sci, Key Lab Photobiol, Inst Bot, Beijing 100093, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China 3.Henan Acad Agr Sci, Inst Farm Prod Proc, Zhengzhou 450002, Peoples R China |
推荐引用方式 GB/T 7714 | Wang, Zhao-Gai,Xu, Tian-Hua,Liu, Cheng,et al. Fast isolation of highly active photosystem ii core complexes from spinach[J]. Journal of integrative plant biology,2010,52(9):793-800. |
APA | Wang, Zhao-Gai,Xu, Tian-Hua,Liu, Cheng,&Yang, Chun-Hong.(2010).Fast isolation of highly active photosystem ii core complexes from spinach.Journal of integrative plant biology,52(9),793-800. |
MLA | Wang, Zhao-Gai,et al."Fast isolation of highly active photosystem ii core complexes from spinach".Journal of integrative plant biology 52.9(2010):793-800. |
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