QQ客服 官方微博 反馈留言

	Fast isolation of highly active photosystem ii core complexes from spinach
	Wang, Zhao-Gai1,2,3; Xu, Tian-Hua1,2; Liu, Cheng1; Yang, Chun-Hong1
刊名	Journal of integrative plant biology
	2010-09-01
卷号	52期号:9页码:793-800
ISSN号	1672-9072
DOI	10.1111/j.1744-7909.2010.00971.x
通讯作者	Yang, chun-hong(yangch@ibcas.ac.cn)
英文摘要	Purification of photosystem ii (psii) core complexes is a time-consuming and low-efficiency process. in order to isolate pure and active psii core complexes in large amounts, we have developed a fast method to isolate highly active monomeric and dimeric psii core complexes from spinach leaves by using sucrose gradient ultracentrifugation. by using a vertical rotor the process was completed significantly faster compared with a swing-out rotor. in order to keep the core complexes in high activity, the whole isolation procedure was performed in the presence of glycine betain and ph at 6.3. the isolated pigment-protein complexes were characterized by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, absorption spectroscopy, 77 k fluorescence spectroscopy and high performance liquid chromatography. our results show that this method is a better choice for quick and efficient isolation of functionally active psii core complexes.
WOS关键词	THYLAKOID MEMBRANES ; EXTRINSIC PROTEINS ; DENSITY GRADIENT ; VERTICAL ROTOR ; BINDING ; CHLAMYDOMONAS ; PURIFICATION ; FLUORESCENCE ; PARTICLES ; DETERGENT
WOS研究方向	Biochemistry & Molecular Biology ; Plant Sciences
WOS类目	Biochemistry & Molecular Biology ; Plant Sciences
语种	英语
出版者	WILEY-BLACKWELL
WOS记录号	WOS:000281216900003
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2408422
专题	中国科学院大学
通讯作者	Yang, Chun-Hong
作者单位	1.Chinese Acad Sci, Key Lab Photobiol, Inst Bot, Beijing 100093, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China 3.Henan Acad Agr Sci, Inst Farm Prod Proc, Zhengzhou 450002, Peoples R China
推荐引用方式 GB/T 7714	Wang, Zhao-Gai,Xu, Tian-Hua,Liu, Cheng,et al. Fast isolation of highly active photosystem ii core complexes from spinach[J]. Journal of integrative plant biology,2010,52(9):793-800.
APA	Wang, Zhao-Gai,Xu, Tian-Hua,Liu, Cheng,&Yang, Chun-Hong.(2010).Fast isolation of highly active photosystem ii core complexes from spinach.Journal of integrative plant biology,52(9),793-800.
MLA	Wang, Zhao-Gai,et al."Fast isolation of highly active photosystem ii core complexes from spinach".Journal of integrative plant biology 52.9(2010):793-800.

个性服务

查看访问统计

相关权益政策

暂无数据

收藏/分享

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。