QQ客服 官方微博 反馈留言

	The membrane protein of severe acute respiratory syndrome coronavirus acts as a dominant immunogen revealed by a clustering region of novel functionally and structurally defined cytotoxic t-lymphocyte epitopes
	Liu, Jun1,2,3; Sun, Yeping1,2,3; Qi, Jianxun1,3; Chu, Fuliang1; Wu, Hao4; Gao, Feng5; Li, Taisheng6,7,8; Yan, Jinghua1,3; Gao, George F.1,2,3,9
刊名	Journal of infectious diseases
	2010-10-15
卷号	202期号:8页码:1171-1180
ISSN号	0022-1899
DOI	10.1086/656315
通讯作者	Gao, george f.(gaof@im.ac.cn)
英文摘要	Background. severe acute respiratory syndrome coronavirus (sars-cov), which emerged with highly contagious and life-threatening characteristics in 2002, remains a potential risk for future outbreaks. membrane (m) and envelope (e) proteins are major structural proteins of the sars-cov. the m protein has been determined as a protective antigen in humoral responses. however, its potential roles in stimulating cellular immunity remain elusive. methods. in this study, a panel of peptides derived from m and e proteins were tested by in vitro refolding, t2 cell-binding assays, and responses stimulated by cytotoxic t-lymphocyte (ctl) epitopes in hla-a2.1/k(b) transgenic mice and human peripheral blood mononuclear cells (pbmcs). results. a nonameric epitope mn2 and a decameric epitope md3 derived from the m protein were identified and used for the evaluation of m protein-specific immunity. responses stimulated by m protein-specific ctl epitopes have been found in the pbmcs of donors who had recovered from sars infection. additionally, the transmembrane domain of the m protein may contain a t cell epitope cluster revealed by the immunogenic and structural analysis of a panel of truncated peptides overlapping with mn2 and md3. conclusions. the m protein of sars-cov holds dominant cellular immunogenicity. this, together with previous reports of a strong humoral response against the m protein, may help to further explain the immunogenicity of sars and serves as potential targets for sars-cov vaccine design.
WOS关键词	RECOVERED SARS PATIENTS ; CELL RESPONSES ; HONG-KONG ; CRYSTAL-STRUCTURES ; TRANSGENIC MICE ; SPIKE PROTEIN ; CTL EPITOPES ; CLASS-I ; IDENTIFICATION ; PEPTIDE
WOS研究方向	Immunology ; Infectious Diseases ; Microbiology
WOS类目	Immunology ; Infectious Diseases ; Microbiology
语种	英语
出版者	OXFORD UNIV PRESS
WOS记录号	WOS:000288250900005
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2407379
专题	中国科学院大学
通讯作者	Gao, George F.
作者单位	1.Chinese Acad Sci, Inst Microbiol, CAS Key Lab Pathogen Microbiol & Immunol, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100101, Peoples R China 3.Chinese Acad Sci, Inst Microbiol, China Japan Joint Lab Mol Immunol & Mol Microbiol, Beijing 100101, Peoples R China 4.Capital Med Univ, You An Hosp, Dept Infect Dis, Beijing, Peoples R China 5.Chinese Acad Sci, Inst Biophys, Beijing 100101, Peoples R China 6.Beijing Union Med Coll Hosp, Dept Infect Dis, Beijing, Peoples R China 7.Chinese Acad Med Sci, AIDS Res Ctr, Beijing 100037, Peoples R China 8.Peking Union Med Coll, Beijing 100021, Peoples R China 9.Chinese Acad Sci, Beijing Inst Life Sci, Beijing 100101, Peoples R China
推荐引用方式 GB/T 7714	Liu, Jun,Sun, Yeping,Qi, Jianxun,et al. The membrane protein of severe acute respiratory syndrome coronavirus acts as a dominant immunogen revealed by a clustering region of novel functionally and structurally defined cytotoxic t-lymphocyte epitopes[J]. Journal of infectious diseases,2010,202(8):1171-1180.
APA	Liu, Jun.,Sun, Yeping.,Qi, Jianxun.,Chu, Fuliang.,Wu, Hao.,...&Gao, George F..(2010).The membrane protein of severe acute respiratory syndrome coronavirus acts as a dominant immunogen revealed by a clustering region of novel functionally and structurally defined cytotoxic t-lymphocyte epitopes.Journal of infectious diseases,202(8),1171-1180.
MLA	Liu, Jun,et al."The membrane protein of severe acute respiratory syndrome coronavirus acts as a dominant immunogen revealed by a clustering region of novel functionally and structurally defined cytotoxic t-lymphocyte epitopes".Journal of infectious diseases 202.8(2010):1171-1180.

个性服务

查看访问统计

相关权益政策

暂无数据

收藏/分享

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。