QQ客服 官方微博 反馈留言

	Effects of dietary protein levels and genetic background on enzyme and transcription factor mrna expression in subcutaneous adipose tissues in swine
	Gu, Wanting1,2,3; Ying, Peng4; Gong, Min1,2,5; Ruan, Zheng1,2; Wang, Yongfei6; Yin, Ronghua6; Shu, Xugang7; Fan, Mingzhe8; Liu, Zhi-qiang3
刊名	Journal of food agriculture & environment
	2010-07-01
卷号	8期号:3-4页码:56-65
关键词	Dietary protein Fattening pigs Ningxiang breed Durocxlandracexyorkshire hybrid Subcutaneous Mrna expression
ISSN号	1459-0255
通讯作者	Ruan, zheng(ezruan@yahoo.com)
英文摘要	The aim of this study was mainly to investigate the effects of dietary crude protein (cp) levels on growth and carcass performance, serum parameter and the mrna expression of enzymes and transcription factors in subcutaneous adipose tissues in obese and lean swine. twenty seven obese-type ningxiang breed fattening pigs and thirty six lean-type hybrid (duroc(x)landrace(x)yorkshire)fattening pigs were randomly allocated to three by two groups, fed low, middle and high cp diets, respectively, for a 46-day period. the cp levels in the diets were 10.41% (low), 12.91% (middle) and 15.43% (high) for ningxiang; 12.91% (low), 15.43% (middle) and 19.66% (high) for the hybrid according to their own standard maintenance requirement, respectively. all diets were identical in calories. on day 46, blood samples were collected for analysis of serum lipoprotein, total cholesterol, triglycerides and leptin levels. following the blood collection, 4 pigs from each group were killed and subcutaneous adipose tissues were collected for the analysis of mrna expression of lipogenic and lipolytic enzymes and transcription factors. results indicated that dietary cp levels had no significant effect on growth and carcass performance, serum parameter and mrna expression of lipogenic and lipolytic key enzymes and transcription factors except leptin and sterol regulatory element-binding protein- 1 c (srebp-1c). increases in dietary cp levels resulted in the increase of leptin and decrease of srebp-1c mrna expression in subcutaneous adipose tissues. however, there were marked differences in growth and carcass performance, serum parameter and mrna expression of lipogenic and lipolytic key enzymes and transcription factors between the two breed. the percentage of fat and feed gain ratio, serum triglycerides, total cholesterol, low density lipoprotein and leptin content and the levels of hormone-sensitive lipase (hsl), lipoprotein lipase (lpl) and peroxisome proliferator-activated receptor-gamma(ppar gamma) mrna in subcutaneous adipose tissues were significantly higher in ningxiang pigs. the average fat thickness was positively correlated with the abundance of hsl, lpl, leptin, ppar gamma and liver x receptor-alpha (lxr alpha) rnrna in subcutaneous adipose tissues. furthermore, leptin was positively correlated with lpl and lxr alpha, and was negatively correlated with srebp-1c of mrna expression in subcutaneous adipose tissues. these findings suggest that obese and lean genotype fattening pigs have distinct manners in lipid metabolism, the ningxiang deposited higher energy than hybrid. some lipid key enzymes (hsl, lpl) and transcription factors (ppar gamma, srebp-1c, lxr alpha) may participate in the regulation of lipid metabolism via leptin signaling. dietary protein may indirectly act on these enzymes and transcription factors to influence the subcutaneous lipid metabolism.
WOS关键词	EARLY-WEANED PIGLETS ; ESCHERICHIA-COLI LIPOPOLYSACCHARIDE ; AMINO-ACID DIGESTIBILITY ; ACANTHOPANAX-SENTICOSUS EXTRACT ; TRUE PHOSPHORUS DIGESTIBILITY ; REGRESSION-ANALYSIS TECHNIQUE ; APPARENT ILEAL DIGESTIBILITY ; TIBETAN SUCKLING PIGLETS ; HORMONE-SENSITIVE LIPASE ; ACTIVATED RECEPTOR-GAMMA
WOS研究方向	Food Science & Technology
WOS类目	Food Science & Technology
语种	英语
出版者	WFL PUBL
WOS记录号	WOS:000286039800008
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2407154
专题	中国科学院大学
通讯作者	Ruan, Zheng
作者单位	1.Nanchang Univ, State Key Lab Food Sci & Technol, Nanchang 330031, Peoples R China 2.Nanchang Univ, Dept Food Sci & Engn, Nanchang 330031, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China 4.Hunan Open Univ, Kaifung 410004, Hunan, Peoples R China 5.Jiangxi Sci & Technol Normal Univ, Nanchang, Jiangxi, Peoples R China 6.Twins Grp Co Ltd, Ctr Res & Dev, Nanchang 330031, Peoples R China 7.Guangzhou Tanke Ind Co Ltd, Guangzhou 510625, Guangdong, Peoples R China 8.Univ Guelph, Dept Anim & Poultry Sci, Guelph, ON N1G 2W15, Canada
推荐引用方式 GB/T 7714	Gu, Wanting,Ying, Peng,Gong, Min,et al. Effects of dietary protein levels and genetic background on enzyme and transcription factor mrna expression in subcutaneous adipose tissues in swine[J]. Journal of food agriculture & environment,2010,8(3-4):56-65.
APA	Gu, Wanting.,Ying, Peng.,Gong, Min.,Ruan, Zheng.,Wang, Yongfei.,...&Liu, Zhi-qiang.(2010).Effects of dietary protein levels and genetic background on enzyme and transcription factor mrna expression in subcutaneous adipose tissues in swine.Journal of food agriculture & environment,8(3-4),56-65.
MLA	Gu, Wanting,et al."Effects of dietary protein levels and genetic background on enzyme and transcription factor mrna expression in subcutaneous adipose tissues in swine".Journal of food agriculture & environment 8.3-4(2010):56-65.

个性服务

查看访问统计

相关权益政策

暂无数据

收藏/分享

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。