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	Molecular cloning and characterization of four heat shock protein genes from macrocentrus cingulum (hymenoptera: braconidae)
	Xu, Pengjun2,3; Xiao, Jinhua2,3; Liu, Li2,3; Li, Tong2,3; Huang, Dawei1,2,4
刊名	Molecular biology reports
	2010-06-01
卷号	37期号:5页码:2265-2272
关键词	Gene expression Heat shock regulation Hsps Phylogenetic Qpcr
ISSN号	0301-4851
DOI	10.1007/s11033-009-9715-z
通讯作者	Huang, dawei(huangdw@ioz.ac.cn)
英文摘要	The full-length cdna sequences of four hsps genes were amplified from macrocentrus cingulum, respectively named mchsp90, mchsc70, mchsp70 and mchsp23.8. these four genes were submitted to genbank database and assigned to genbank accession number: eu570065, eu585780, eu585779 and eu624206. phylogenetic analysis indicated that the mchsp90, mchsc70 and mchsp70 were located in cytoplasm. moreover, the mchsc70 was a member of heat shock cognate protein 70 while the mchsp70 was a member of heat shock inducible 70. the expression analysis indicated that these four genes were differentially expressed at larval, pupal and adult stages. under normal conditions, the mrna levels of mchsp90 and mchsc70 were higher than mchsp70 while mchsp23.8 was decreased during aging. the mrna levels of these four genes could be up-regulated by heat shock besides mchsp70 and mchsp23.8 could be increased more dramatically than mchsp90 and mchsc70. these results suggested that mchsp90, mchsp70 and mchsp23.8 might have dual functions under normal and heat shock conditions while mchsp70 and mchsp23.8 might supply more important protection under heat shock.
WOS关键词	HEAT-SHOCK-PROTEIN-70 FAMILY ; DEVELOPMENTAL EXPRESSION ; SEQUENCE ALIGNMENT ; PUPAL DIAPAUSE ; UP-REGULATION ; CDNA CLONING ; FLESH FLY ; EVOLUTION ; HSP90 ; HSP70
WOS研究方向	Biochemistry & Molecular Biology
WOS类目	Biochemistry & Molecular Biology
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000279308400017
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2406404
专题	中国科学院大学
通讯作者	Huang, Dawei
作者单位	1.Chinese Acad Sci, Inst Zool, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Inst Zool, Key Lab Zool Systemat & Evolut, Beijing 100101, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China 4.Shandong Agr Univ, Coll Plant Protect, Tai An 271018, Shandong, Peoples R China
推荐引用方式 GB/T 7714	Xu, Pengjun,Xiao, Jinhua,Liu, Li,et al. Molecular cloning and characterization of four heat shock protein genes from macrocentrus cingulum (hymenoptera: braconidae)[J]. Molecular biology reports,2010,37(5):2265-2272.
APA	Xu, Pengjun,Xiao, Jinhua,Liu, Li,Li, Tong,&Huang, Dawei.(2010).Molecular cloning and characterization of four heat shock protein genes from macrocentrus cingulum (hymenoptera: braconidae).Molecular biology reports,37(5),2265-2272.
MLA	Xu, Pengjun,et al."Molecular cloning and characterization of four heat shock protein genes from macrocentrus cingulum (hymenoptera: braconidae)".Molecular biology reports 37.5(2010):2265-2272.

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