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	Cdna cloning and expression pattern of two enolase genes from the chinese oak silkworm, antheraea pernyi
	Liu, Yanqun1,2; Li, Yuping1; Wang, Huan1; Xia, Runxi1; Li, Xisheng2; Wan, Haolei3,4; Qin, Li1; Jiang, Defu2; Lu, Cheng5; Xiang, Zhonghuai5
刊名	Acta biochimica et biophysica sinica
	2010-11-01
卷号	42期号:11页码:816-826
关键词	Antheraea pernyi Enolase Cloning Expression pattern
ISSN号	1672-9145
DOI	10.1093/abbs/gmq084
通讯作者	Liu, yanqun(liuyqlyp@yahoo.com.cn)
英文摘要	In this study, two enolase genes were isolated and characterized from the chinese oak silkworm, antheraea perny, which were designated as enolase i and ii, respectively. the enolase i cdna sequence was 1712 bp with an open reading frame (orf) of 1302 bp encoding 433 amino acids. the enolase ii cdna sequence was 1549 bp with an orf of 1296 bp encoding 431 amino acids. the amino acid sequences of the two genes share several conserved features/sites of enolase. antheraea pernyi enolase i shows 93%-97% sequence identity to enolases of lepidopterans available to date, 75%-82% identity to enolases of other invertebrates, 60%-72% identity to enolases of other organisms including vertebrates, plants, and fungi. antheraea pernyi enolase ii shows 84% identity to bombyx mori enolase ii, but 60% identity to a. pernyi enolase i. in the phylogenetic tree, enolase ii sequences from a. pernyi and b. mori were clearly separated from the majority of enolase sequences of higher organisms including a. pernyi and b. mori enolase i sequences. by sequence comparisons and phylogenetic analysis, we suggest that enolase ii from a. pernyi and b. mori may be a new member of the enolase superfamily. antheraea pernyi enolase i mrna was found in all tested tissues whereas enolase ii mrna was expressed specifically in the spermaries and ovaries, suggesting that the product of enolase ii gene may be related to reproduction. the transcript abundance of a. pernyi enolase i gene was significantly down-regulated after cold shock and significantly up-regulated after heat shock, suggesting that a. pernyi enolase i gene may be inducible by temperature stress.
WOS关键词	HUMAN ALPHA-ENOLASE ; NUCLEOTIDE-SEQUENCE ; MOLECULAR-CLONING ; GENOME SEQUENCE ; ANGSTROM RESOLUTION ; CRYSTAL-STRUCTURE ; BOMBYX-MORI ; PROTEIN ; EVOLUTION ; STRESS
WOS研究方向	Biochemistry & Molecular Biology ; Biophysics
WOS类目	Biochemistry & Molecular Biology ; Biophysics
语种	英语
出版者	OXFORD UNIV PRESS
WOS记录号	WOS:000283657600008
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2405620
专题	中国科学院大学
通讯作者	Liu, Yanqun
作者单位	1.Shenyang Agr Univ, Coll Biosci & Biotechnol, Dept Sericulture, Shenyang 110866, Peoples R China 2.Sericultural Inst Liaoning Prov, Fengcheng 118100, Peoples R China 3.Chinese Acad Sci, Beijing Inst Genom, CAS Key Lab Genome Sci & Informat, Beijing 100029, Peoples R China 4.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China 5.Southwest Univ, Minist Agr, Key Sericultural Lab, Chongqing 400716, Peoples R China
推荐引用方式 GB/T 7714	Liu, Yanqun,Li, Yuping,Wang, Huan,et al. Cdna cloning and expression pattern of two enolase genes from the chinese oak silkworm, antheraea pernyi[J]. Acta biochimica et biophysica sinica,2010,42(11):816-826.
APA	Liu, Yanqun.,Li, Yuping.,Wang, Huan.,Xia, Runxi.,Li, Xisheng.,...&Xiang, Zhonghuai.(2010).Cdna cloning and expression pattern of two enolase genes from the chinese oak silkworm, antheraea pernyi.Acta biochimica et biophysica sinica,42(11),816-826.
MLA	Liu, Yanqun,et al."Cdna cloning and expression pattern of two enolase genes from the chinese oak silkworm, antheraea pernyi".Acta biochimica et biophysica sinica 42.11(2010):816-826.

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