| Drosophila twinfilin is required for cell migration and synaptic endocytosis | |
| Wang, Dan3,4; Zhang, Lijun1,2; Zhao, Guoli3,4; Wahlstrom, Gudrun5,6; Heino, Tapio I.5,6; Chen, Jiong1,2; Zhang, Yong Q.3,4 | |
| 刊名 | Journal of cell science
 |
| 2010-05-01 | |
| 卷号 | 123期号:9页码:1546-1556 |
| 关键词 | Twinfilin Actin turnover Cell migration Synapse Endocytosis |
| ISSN号 | 0021-9533 |
| DOI | 10.1242/jcs.060251 |
| 通讯作者 | Chen, jiong(chenjiong@nicemice.cn) |
| 英文摘要 | Precise actin regulation is essential for diverse cellular processes such as axonal growth, cell migration and endocytosis. twinfilin (twf) encodes a protein that sequesters actin monomers, but its in vivo functions are unclear. in this study, we characterized twf-null mutants in a metazoan for the first time and found that drosophila twf negatively regulates f-actin formation in subcellular regions of rapid actin turnover in three different systems, namely postsynaptic neuromuscular junction (nmj) synapses, migratory border cells and epithelial follicle cells. loss of twf function results in defects in axonal growth in the brain and border cell migration in the ovary. additionally, we found that the actin-dependent postsynaptic localization of glutamate receptor gluriia, but not gluriib, was specifically reduced in twf mutants. more importantly, we showed that twf mutations caused significantly reduced presynaptic endocytosis at nmj synapses, as detected using the fluorescent dye fm1-43 uptake assay. furthermore, electrophysiological analysis under high-frequency stimulation showed compromised neurotransmission in twf mutant synapses, confirming an insufficient replenishment of synaptic vesicles. together, our results reveal that twinfilin promotes actin turnover in multiple cellular processes that are highly dependent on actin dynamics. |
| WOS关键词 | CLATHRIN-MEDIATED ENDOCYTOSIS ; ACTIN CYTOSKELETON ; NEUROMUSCULAR-JUNCTION ; CAPPING PROTEIN ; IN-VIVO ; BINDING PROTEINS ; MONOMERS ; RECEPTOR ; DYNAMICS ; PROFILIN |
| WOS研究方向 | Cell Biology |
| WOS类目 | Cell Biology |
| 语种 | 英语 |
| 出版者 | COMPANY OF BIOLOGISTS LTD |
| WOS记录号 | WOS:000276912300019 |
| 内容类型 | 期刊论文 |
| URI标识 | http://www.corc.org.cn/handle/1471x/2405608 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Chen, Jiong |
| 作者单位 | 1.Nanjing Univ, Model Anim Res Ctr, Nanjing 210061, Peoples R China 2.Nanjing Univ, MOE Key Lab Model Anim Dis Study, Nanjing 210061, Peoples R China 3.Chinese Acad Sci, Inst Genet & Dev Biol, Key Lab Mol & Dev Biol, Beijing 100101, Peoples R China 4.Chinese Acad Sci, Grad Univ, Beijing 100101, Peoples R China 5.Univ Helsinki, Inst Biotechnol, FIN-00014 Helsinki, Finland 6.Univ Helsinki, Dept Biosci, FIN-00014 Helsinki, Finland |
| 推荐引用方式 GB/T 7714 | Wang, Dan,Zhang, Lijun,Zhao, Guoli,et al. Drosophila twinfilin is required for cell migration and synaptic endocytosis[J]. Journal of cell science,2010,123(9):1546-1556. |
| APA | Wang, Dan.,Zhang, Lijun.,Zhao, Guoli.,Wahlstrom, Gudrun.,Heino, Tapio I..,...&Zhang, Yong Q..(2010).Drosophila twinfilin is required for cell migration and synaptic endocytosis.Journal of cell science,123(9),1546-1556. |
| MLA | Wang, Dan,et al."Drosophila twinfilin is required for cell migration and synaptic endocytosis".Journal of cell science 123.9(2010):1546-1556. |
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