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	Enhancement of sensitivity and specificity of the fluoroimmunoassay of hepatitis b virus surface antigen through "flexible" coupling between quantum dots and antibody
	Zeng, Qinghui1,2; Zhang, Youlin1,3; Song, Kai1; Kong, Xianggui1; Aalders, Maurice C. G.4; Zhang, Hong3
刊名	Talanta
	2009-11-15
卷号	80期号:1页码:307-312
关键词	Cdte/cds core/shell quantum dots Protein g Flexible coupling Fluorescent immune detection Specificity
ISSN号	0039-9140
DOI	10.1016/j.talanta.2009.06.061
通讯作者	Kong, xianggui(xgkong14@ciomp.ac.cn)
英文摘要	Quantum dots (qds) are widely used in the immune detection. yet, the sensitivity and specificity of the immune detection are not satisfactory because the binding sites of qds onto antibody (ab) are often arbitrary and the influence of the large surface electronic potential energy of qds on the directly conjugated ab is nonnegligible. in this work, we provide a "flexible" coupling method, in which protein g (pg) is selected as the flexible bridge between the qds and the hepatitis b virus surface antibody (hbsab), to improve the sensitivity and specificity of the fluoroimmunoassay compared to the directly covalent conjugation. successful coupling of the hbsab to our highly luminescent cdte/cds core/shell qds is proven with gel electrophoresis and atomic force microscopy (afm). the assay results, based on the microelisa well plate as matrix to immobilize the sandwich structure, show that both sensitivity and specificity can be improved greatly through the flexible coupled qds-pg-ab conjugates. (c) 2009 published by elsevier b.v.
WOS关键词	ATOMIC-FORCE MICROSCOPY ; IN-VIVO ; NANOCRYSTALS ; PROTEIN ; CDTE ; CONJUGATION ; CDSE
WOS研究方向	Chemistry
WOS类目	Chemistry, Analytical
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000271055700046
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2404920
专题	中国科学院大学
通讯作者	Kong, Xianggui
作者单位	1.Chinese Acad Sci, Changchun Inst Opt Fine Mech & Phys, Key Lab Excited State Proc, Changchun 130033, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China 3.Univ Amsterdam, Vant Hoff Inst Mol Sci, NL-1018 WV Amsterdam, Netherlands 4.Univ Amsterdam, Acad Med Ctr, Dept Biomed Engn & Phys, NL-1105 AZ Amsterdam, Netherlands
推荐引用方式 GB/T 7714	Zeng, Qinghui,Zhang, Youlin,Song, Kai,et al. Enhancement of sensitivity and specificity of the fluoroimmunoassay of hepatitis b virus surface antigen through "flexible" coupling between quantum dots and antibody[J]. Talanta,2009,80(1):307-312.
APA	Zeng, Qinghui,Zhang, Youlin,Song, Kai,Kong, Xianggui,Aalders, Maurice C. G.,&Zhang, Hong.(2009).Enhancement of sensitivity and specificity of the fluoroimmunoassay of hepatitis b virus surface antigen through "flexible" coupling between quantum dots and antibody.Talanta,80(1),307-312.
MLA	Zeng, Qinghui,et al."Enhancement of sensitivity and specificity of the fluoroimmunoassay of hepatitis b virus surface antigen through "flexible" coupling between quantum dots and antibody".Talanta 80.1(2009):307-312.

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