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	Discovery of immune-related genes in chinese mitten crab (eriocheir sinensis) by expressed sequence tag analysis of haemocytes
	Zhao, Daxian1; Song, Shuhui2,3; Wang, Qun1; Zhang, Xiaowei2; Hu, Songnian2; Chen, Liqiao1
刊名	Aquaculture
	2009-02-18
卷号	287期号:3-4页码:297-303
关键词	Eriocheir sinensis Haemocyte Express sequence tag Immune-related gene
ISSN号	0044-8486
DOI	10.1016/j.aquaculture.2008.10.050
通讯作者	Chen, liqiao(lqchen@bio.ecnu.edu.cn)
英文摘要	To identify distinctive genes associated with immunity in the chinese mitten crab (eriocheir sinensis), an expressed sequence tag (est) library was constructed from haemocytes of this economically important species. 3118 clones were unidirectionally sequenced and analyzed by homology searches against sequences in the genbank, kegg and uniprot. significant homology (blastx, e-values <10(-5)) to known genes was found in 488 of teh 1039 unique sequences. the automatic functional classification based on keff and gene ontology revealed 26 putative immune-realted genes(2.5% of all unique sequences, 18.9% of all ests). these 26 genes coded for enzymes and proteins in the clotting and prophenoloxidase-activating system, antioxidative enzymes, antimicrobial peptides, and pattern recognition molecules. the existence of these molecular processes in the activation of cellular defense in crabs has not been previously reported. according to est abundance, the major immune-related genes were the kazal-type proteinase inhibitor (337, 11.1% of all ests) and the c-type lectin (72, 2.4%). the est sequences of mitten crab haemocytes provide important information for understanding the evolution of the immune system among crustaceans in general. this study lays the groundwork for development of molecular markers related to disease resistance in the crab.
WOS关键词	BLACK TIGER SHRIMP ; FRESH-WATER CRAYFISH ; BETA-1,3-GLUCAN BINDING-PROTEIN ; PRAWN MARSUPENAEUS-JAPONICUS ; HEMOLYMPH CLOTTABLE PROTEINS ; PACIFIC WHITE SHRIMP ; C-TYPE LECTIN ; PENAEUS-MONODON ; MOLECULAR-CLONING ; FENNEROPENAEUS-CHINENSIS
WOS研究方向	Fisheries ; Marine & Freshwater Biology
WOS类目	Fisheries ; Marine & Freshwater Biology
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000263388500010
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2404725
专题	中国科学院大学
通讯作者	Chen, Liqiao
作者单位	1.E China Normal Univ, Dept Biol, Shanghai 200062, Peoples R China 2.Chinese Acad Sci, Beijing Inst Genom, Key Lab Genome Sci & Informat, Beijing 100029, Peoples R China 3.Chinese Acad Sci, Grad Univ, Beijing 100094, Peoples R China
推荐引用方式 GB/T 7714	Zhao, Daxian,Song, Shuhui,Wang, Qun,et al. Discovery of immune-related genes in chinese mitten crab (eriocheir sinensis) by expressed sequence tag analysis of haemocytes[J]. Aquaculture,2009,287(3-4):297-303.
APA	Zhao, Daxian,Song, Shuhui,Wang, Qun,Zhang, Xiaowei,Hu, Songnian,&Chen, Liqiao.(2009).Discovery of immune-related genes in chinese mitten crab (eriocheir sinensis) by expressed sequence tag analysis of haemocytes.Aquaculture,287(3-4),297-303.
MLA	Zhao, Daxian,et al."Discovery of immune-related genes in chinese mitten crab (eriocheir sinensis) by expressed sequence tag analysis of haemocytes".Aquaculture 287.3-4(2009):297-303.

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