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	[ru(bpy)(2)dppz](2+) electrochemiluminescence switch and its applications for dna interaction study and label-free atp aptasensor
	Hu, Lianzhe1,2; Bian, Zheng3; Li, Haijuan1,2; Han, Shuang1,2; Yuan, Yali1,2; Gao, Lianxun3; Xu, Guobao1
刊名	Analytical chemistry
	2009-12-01
卷号	81期号:23页码:9807-9811
ISSN号	0003-2700
DOI	10.1021/ac901925x
通讯作者	Xu, guobao(guobaoxu@ciac.jl.cn)
英文摘要	[ru(bpy)(2)dppz](2+) electrochemilumineseence (ecl) was studied, and it was used to investigate dna interaction and develop a label-free atp aptasensor for the first time. ecl of [ru(bpy)(2)dppz](2+) is negligible in aqueous solution, and increases similar to 1000 times when [ru(bpy)(2)dppz](2+) intercalates into the nucleic acid structure. the ecl switch behavior of [ru(bpy)(2)dppz](2+) is ascribed to the intercalation that shields the phenazine nitrogens from the solvent and results in a luminescent excited state. the ecl switch by dna was applied to investigate the interaction of [ru(bpy)(2)dppz](2+) with herring sperm dna. the calculated equilibrium constant (k) is 1.35 x 10(6) m-1, and the calculated binding-site size (s) is 0.88 base pair, which is consistent with the reported values. moreover, atp can dramatically affect ecl of the [ru(bpy)(2)dppz](2+)/atp aptamer complex. as a result, a label-free, sensitive, and selective [ru(bpy)(2)dppz](2+) ecl method for atp detection was developed. the detection limit is 100 nm for atp (with a signal-to-noise ratio, s/n, of 3) with a linear range of 0-1 mu m. ne result demonstrates that [ru(bpy)(2)dppz](2+) ecl holds great promise in aptasensors.
WOS关键词	MOLECULAR LIGHT SWITCH ; ELECTROGENERATED CHEMILUMINESCENCE ; DIPYRIDOPHENAZINE COMPLEXES ; APTAMER ; RUTHENIUM ; AMPLIFICATION ; SENSITIVITY ; BINDING ; SYSTEM ; ECL
WOS研究方向	Chemistry
WOS类目	Chemistry, Analytical
语种	英语
出版者	AMER CHEMICAL SOC
WOS记录号	WOS:000272084700043
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2404596
专题	中国科学院大学
通讯作者	Xu, Guobao
作者单位	1.Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Electroanalyt Chem, Changchun 130022, Jilin, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100864, Peoples R China 3.Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Polymer Phys & Chem, Changchun 130022, Jilin, Peoples R China
推荐引用方式 GB/T 7714	Hu, Lianzhe,Bian, Zheng,Li, Haijuan,et al. [ru(bpy)(2)dppz](2+) electrochemiluminescence switch and its applications for dna interaction study and label-free atp aptasensor[J]. Analytical chemistry,2009,81(23):9807-9811.
APA	Hu, Lianzhe.,Bian, Zheng.,Li, Haijuan.,Han, Shuang.,Yuan, Yali.,...&Xu, Guobao.(2009).[ru(bpy)(2)dppz](2+) electrochemiluminescence switch and its applications for dna interaction study and label-free atp aptasensor.Analytical chemistry,81(23),9807-9811.
MLA	Hu, Lianzhe,et al."[ru(bpy)(2)dppz](2+) electrochemiluminescence switch and its applications for dna interaction study and label-free atp aptasensor".Analytical chemistry 81.23(2009):9807-9811.

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