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	Synthesis, chromatographic evaluation and hydrophilic interaction/reversed-phase mixed-mode behavior of a "click beta-cyclodextrin" stationary phase
	Guo, Zhimou1; Jin, Yu1,2; Liang, Tu1; Liu, Yanfang1; Xu, Qing1; Liang, Xinmiao1,2; Lei, Aiwen3
刊名	Journal of chromatography a
	2009-01-09
卷号	1216期号:2页码:257-263
关键词	Beta-cyclodextrin Stationary phase Click chemistry Hydrophilic interaction liquid chromatography Polar components Mixed-mode stationary phase
ISSN号	0021-9673
DOI	10.1016/j.chroma.2008.11.071
通讯作者	Liang, xinmiao(liangxm@dicp.ac.cn)
英文摘要	A native beta-cyclodextrin (beta-cd) stationary phase was prepared by covalently bonding beta-cd on silica particles via huisgen [3 + 2] dipolar cycloaddition between the organic azide and terminal alkyne, the so-called click chemistry. the resulting beta-cd bonded silica (click beta-cd) was characterized by ft-ir, solid state c-13 cross polarization/magic-angle spinning (cp/mas) nmr and elemental analyses. which proved the successful immobilization of beta-cd on the silica support with click chemistry. the retentive properties of click beta-cid were investigated under hydrophilic interaction liquid chromatography (hilic) mode in different mobile phase conditions with a set of polar compounds including nucleosides, organic acids and alkaloids. the effects of water content, concentration of the salt and ph of the buffer solution on the retention time were studied and the results demonstrated the typical retention behavior of hilic on click beta-cd. separation of very polar components, such as nucleosides and oligosaccharides, and chiral separation under hilic mode were successfully achieved. in addition, click beta-cd was chromatographically evaluated with a set of flavone glycosides. the retention curves depending on the mobile phase of acetonitrile content were "u" curves. which is an indication of hilic/rplc mixed-mode retention behavior. the difference of the separation selectivity between hilic and rplc was described as orthogonality by using geometric approach and the orthogonality reached 69.4%. the mixed-mode hplc properties and excellent orthogonality demonstrated the flexibility in hplc methods development and great potential in two-dimensional liquid chromatography separation. (c) 2008 elsevier b.v. all rights reserved.
WOS关键词	PERFORMANCE LIQUID-CHROMATOGRAPHY ; POLAR COMPOUNDS ; SILICA-GEL ; MASS-SPECTROMETRY ; REVERSED-PHASE ; ADRENORECEPTOR AGONISTS ; RETENTION PREDICTION ; SEPARATION MATERIALS ; CHEMISTRY ; HPLC
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
WOS类目	Biochemical Research Methods ; Chemistry, Analytical
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000262697100010
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2401068
专题	中国科学院大学
通讯作者	Liang, Xinmiao
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, Grad Sch, Dalian 116023, Peoples R China 2.E China Univ Sci & Technol, Sch Pharm, Shanghai 200237, Peoples R China 3.Wuhan Univ, Coll Chem & Mol Sci, Wuhan 430072, Peoples R China
推荐引用方式 GB/T 7714	Guo, Zhimou,Jin, Yu,Liang, Tu,et al. Synthesis, chromatographic evaluation and hydrophilic interaction/reversed-phase mixed-mode behavior of a "click beta-cyclodextrin" stationary phase[J]. Journal of chromatography a,2009,1216(2):257-263.
APA	Guo, Zhimou.,Jin, Yu.,Liang, Tu.,Liu, Yanfang.,Xu, Qing.,...&Lei, Aiwen.(2009).Synthesis, chromatographic evaluation and hydrophilic interaction/reversed-phase mixed-mode behavior of a "click beta-cyclodextrin" stationary phase.Journal of chromatography a,1216(2),257-263.
MLA	Guo, Zhimou,et al."Synthesis, chromatographic evaluation and hydrophilic interaction/reversed-phase mixed-mode behavior of a "click beta-cyclodextrin" stationary phase".Journal of chromatography a 1216.2(2009):257-263.

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