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	Proteomics analysis of drought stress-responsive proteins in hippophae rhamnoides l.
	Xu, Gang2,3,4; Li, Chunyang3,4; Yao, Yinan1
刊名	Plant molecular biology reporter
	2009-06-01
卷号	27期号:2页码:153-161
关键词	Drought Two-dimensional gel electrophoresis Hippophae rhamnoides l. Mass spectrometry
ISSN号	0735-9640
DOI	10.1007/s11105-008-0067-y
通讯作者	Yao, yinan(yaoyinan0430@163.com)
英文摘要	Hippophae rhamnoides l. is uniquely capable of growing well under extreme environmental conditions such as water deficit, low temperature, and high altitude. such tolerance invokes much interest in understanding the biology of this plant species and its utilization potential. in this study, analysis of drought stress-responsive proteins in h. rhamnoides was conducted wherein greenhouse-grown seedlings were subjected to drought stress. by using proteomic techniques, proteins, extracted from leaves, were analyzed using two-dimensional electrophoresis and maldi-tof ms. altogether, 55 proteins exhibited changes in abundance under stress. of these, 13 proteins were identified, including three that disappeared under drought (a putative abc transporter atp-binging protein, a heat shock protein hs1u, and a hypothetical protein xp515578), seven that were up-regulated (three large subunits of rubisco, a hypothetical protein dsm3645-23351, a putative acyl-coa dehydrogenase, a nesprin-2, and a j-type co-chaperone hsc20), and three that were only detected under drought (a probable nitrogen regulation protein (ntrx), a 4-hydroxyphenylpyruvate dioxygenase, and an unnamed protein product). these proteins may function in beta-oxidation pathways in mitochondria, across membranes transport, abnormal protein removal, or prevent protein aggregation arrest, cell division, cytoskeleton stabilization, iron-sulfur cluster assembly, nitrogen metabolism regulation, and antioxidant substance biosynthesis. four proteins (j-type co-chaperone hsc20, a putative abc transporter atp-binging protein, ntrx, and hs1u) were deemed as new discoveries in higher plants, and their functions were predicted either from their conserved domains or homologies to other organisms. these results provide new insights into our understanding of the mechanism of drought tolerance in plants.
WOS关键词	NITRATE REDUCTASE-ACTIVITY ; SYNECHOCYSTIS SP PCC-6803 ; NITROGEN-FIXATION ; ESCHERICHIA-COLI ; PHYSIOLOGICAL-RESPONSES ; ABC TRANSPORTERS ; WATER DEFICITS ; MAIZE LEAVES ; ARABIDOPSIS ; PLANT
WOS研究方向	Biochemistry & Molecular Biology ; Plant Sciences
WOS类目	Biochemical Research Methods ; Plant Sciences
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000270805900006
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2398651
专题	中国科学院大学
通讯作者	Yao, Yinan
作者单位	1.Guizhou Univ, Coll Agr, Guiyang 550025, Peoples R China 2.Guizhou Univ, Key Lab Green Pesticide & Agr Bioengn, Chinese Minist Educ, Ctr Res & Dev Fine Chem, Guiyang 550025, Peoples R China 3.Chinese Acad Sci, Chengdu Inst Biol, Chengdu 610041, Peoples R China 4.Chinese Acad Sci, China Grad Sch, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	Xu, Gang,Li, Chunyang,Yao, Yinan. Proteomics analysis of drought stress-responsive proteins in hippophae rhamnoides l.[J]. Plant molecular biology reporter,2009,27(2):153-161.
APA	Xu, Gang,Li, Chunyang,&Yao, Yinan.(2009).Proteomics analysis of drought stress-responsive proteins in hippophae rhamnoides l..Plant molecular biology reporter,27(2),153-161.
MLA	Xu, Gang,et al."Proteomics analysis of drought stress-responsive proteins in hippophae rhamnoides l.".Plant molecular biology reporter 27.2(2009):153-161.

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