| Identification and characterization of two gcva genes in sinorhizobium meliloti | |
| Qi MingSheng1,2; Luo Li1; Cheng HaiPing3; Zhu Jiabi1; Yu GuanQiao1 | |
| 刊名 | Chinese science bulletin
 |
| 2009-02-01 | |
| 卷号 | 54期号:3页码:405-411 |
| 关键词 | Gcva Gcvthp operon Lysr family transcriptional regulator Sinorhizobium meliloti |
| ISSN号 | 1001-6538 |
| DOI | 10.1007/s11434-008-0516-x |
| 通讯作者 | Yu guanqiao(gqyu@sippe.ac.cn) |
| 英文摘要 | Gcva is a member of the lysr family of transcriptional regulators that mediates the expression of the glycine cleavage (gcv) operon (gcvthp) in response to glycine in escherichia coli. in our previous work, 90 putative regulator genes of the lysr family in sinorhizobium meliloti were mutagenized to determine their phenotype. in the present study, we found that the s. meliloti genome had two gcva genes, gcva1 and gcva2. both gcva1 and gcva2 were required for full activation of the gcvthp operon in the presence of exogenous glycine. the gcva1-mediated activation of gcvthp operon was glycine-inducible, while gcva2-mediated activation was not. we speculate that the regulatory mechanism for gcvthp expression in s. meliloti differed from e. coli. evolutionary analysis showed that gcva were distributed in many genera of proteobacteria and the distances between gcva1 and gcva2 in s. meliloti and gcva in e. coli were large, which may explain the different regulatory mechanisms for gcvthp expression. these findings could provide new clues to the role of the lysr gene family. |
| WOS关键词 | CLEAVAGE ENZYME-SYSTEM ; ESCHERICHIA-COLI ; GLYCINE CLEAVAGE ; RHIZOBIUM-MELILOTI ; TRANSCRIPTIONAL REGULATORS ; MEDIATED ACTIVATION ; LYSR FAMILY ; DNA-BINDING ; MUTANTS ; EXOPOLYSACCHARIDE |
| WOS研究方向 | Science & Technology - Other Topics |
| WOS类目 | Multidisciplinary Sciences |
| 语种 | 英语 |
| 出版者 | SCIENCE PRESS |
| WOS记录号 | WOS:000263357000012 |
| 内容类型 | 期刊论文 |
| URI标识 | http://www.corc.org.cn/handle/1471x/2398273 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Yu GuanQiao |
| 作者单位 | 1.Chinese Acad Sci, Mol Genet Lab, Shanghai Inst Plant Physiol & Ecol, Shanghai Inst Biol Sci, Shanghai 200032, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China 3.CUNY Herbert H Lehman Coll, Dept Biol Sci, Bronx, NY 10468 USA |
| 推荐引用方式 GB/T 7714 | Qi MingSheng,Luo Li,Cheng HaiPing,et al. Identification and characterization of two gcva genes in sinorhizobium meliloti[J]. Chinese science bulletin,2009,54(3):405-411. |
| APA | Qi MingSheng,Luo Li,Cheng HaiPing,Zhu Jiabi,&Yu GuanQiao.(2009).Identification and characterization of two gcva genes in sinorhizobium meliloti.Chinese science bulletin,54(3),405-411. |
| MLA | Qi MingSheng,et al."Identification and characterization of two gcva genes in sinorhizobium meliloti".Chinese science bulletin 54.3(2009):405-411. |
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