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	Covalent immobilization of glucose oxidase onto poly(st-gma-nass) monodisperse microspheres via bsa as spacer arm
	Du, Tanxin1,2; Liu, Bailing1; Hou, Xiaohui1,2; Zhang, Baotan1,2; Du, Cuiming1,2
刊名	Applied surface science
	2009-06-30
卷号	255期号:18页码:7937-7941
关键词	Covalent immobilization Bsa-spacer Conformational change Monodisperse microspheres
ISSN号	0169-4332
DOI	10.1016/j.apsusc.2009.04.172
通讯作者	Liu, bailing(blliuchem@hotmail.com)
英文摘要	In this study, the immobilization of glucose oxidase (god) onto micron-size monodisperse poly(styrene-glycidyl methacrylate-sodium sulfonate styrene) microspheres was investigated. in order to improve their surface biocompatibility, bovine serum albumin (bsa) was introduced onto the microspheres' surface as a new type of spacer arm. both the immobilization amount and enzymatic activity were determined in different experimental conditions (enzyme concentration, ph, temperature, etc.). it was found that bsa could serve as a good spacer because it increases both the immobilization amount and enzymatic activity of god. the tolerance of immobilized god against ph, temperatures was examined. moreover, the kinetic parameters for native and immobilized god were obtained and compared. (c) 2009 elsevier b. v. all rights reserved.
WOS关键词	DISPERSION POLYMERIZATION ; TRYPSIN ; MODEL ; BEADS
WOS研究方向	Chemistry ; Materials Science ; Physics
WOS类目	Chemistry, Physical ; Materials Science, Coatings & Films ; Physics, Applied ; Physics, Condensed Matter
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000267469500015
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2395087
专题	中国科学院大学
通讯作者	Liu, Bailing
作者单位	1.Chinese Acad Sci, Chengdu Inst Organ Chem, Chengdu 610041, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	Du, Tanxin,Liu, Bailing,Hou, Xiaohui,et al. Covalent immobilization of glucose oxidase onto poly(st-gma-nass) monodisperse microspheres via bsa as spacer arm[J]. Applied surface science,2009,255(18):7937-7941.
APA	Du, Tanxin,Liu, Bailing,Hou, Xiaohui,Zhang, Baotan,&Du, Cuiming.(2009).Covalent immobilization of glucose oxidase onto poly(st-gma-nass) monodisperse microspheres via bsa as spacer arm.Applied surface science,255(18),7937-7941.
MLA	Du, Tanxin,et al."Covalent immobilization of glucose oxidase onto poly(st-gma-nass) monodisperse microspheres via bsa as spacer arm".Applied surface science 255.18(2009):7937-7941.

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