| The immobilization of proteins on biodegradable fibers via biotin-streptavidin bridges | |
| Lu, Tiancheng1,2; Chen, Xuesi1; Shi, Quan1,2; Wang, Yu1; Zhang, Peibiao1; Jing, Xiabin1 | |
| 刊名 | Acta biomaterialia
 |
| 2008-11-01 | |
| 卷号 | 4期号:6页码:1770-1777 |
| 关键词 | Electrospun fibers Protein immobilization Immunoassay Antibodies Antigens |
| ISSN号 | 1742-7061 |
| DOI | 10.1016/j.actbio.2008.05.006 |
| 通讯作者 | Jing, xiabin(xbjing@ciac.jl.cn) |
| 英文摘要 | This paper aims at developing novel bioactive fibrous mats for protein immobilization and for protein separation/purification. for this purpose, an amphiphilic triblock copolymer, biotinylated poly(ethylene glycol)-b-poly(l-lactide)-b-poly(l-lysine) was co-electrospun together with poly(l-lactide-co-glycolide) into ultrafine fibers similar to 2 mu m in diameter, and a layer of blocking agent was coated on the fiber surfaces to block off possible non-specific binding of proteins. the biotin species retained their ability to specifically recognize and bind streptavidin, and the immobilized streptavidin could further combine with biotinylated antibodies, antigens and other biological moieties. horseradish peroxidase-labeled streptavidin and fluorescein isothiocyanate-labeled goat globulin were used to detect the immobilizations of streptavidin and rabbit anti-goat igg(h + l) via enzyme-linked immunoassay and confocal laser scanning microscope, respectively. the immobilized antigen was eluted from the fiber substrate with a glycine/hcl solution and the eluted antigen retained its bioactivity. therefore, these biotin-carrying composite fibers have a variety of uses, including selective immobilization of functional proteins, antigen/antibody separation and purification, and vaccine preparation. (c) 2008 acta materialia inc. published by elsevier ltd. all rights reserved. |
| WOS关键词 | GLUCOSE-OXIDASE ; BIOSENSOR APPLICATIONS ; ELECTROSPUN ; ENCAPSULATION ; ACID ; STABILITY ; MEMBRANES ; LIPASE ; AVIDIN |
| WOS研究方向 | Engineering ; Materials Science |
| WOS类目 | Engineering, Biomedical ; Materials Science, Biomaterials |
| 语种 | 英语 |
| 出版者 | ELSEVIER SCI LTD |
| WOS记录号 | WOS:000261253400021 |
| 内容类型 | 期刊论文 |
| URI标识 | http://www.corc.org.cn/handle/1471x/2392736 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Jing, Xiabin |
| 作者单位 | 1.Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Polymer Phys & Chem, Changchun 130022, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Lu, Tiancheng,Chen, Xuesi,Shi, Quan,et al. The immobilization of proteins on biodegradable fibers via biotin-streptavidin bridges[J]. Acta biomaterialia,2008,4(6):1770-1777. |
| APA | Lu, Tiancheng,Chen, Xuesi,Shi, Quan,Wang, Yu,Zhang, Peibiao,&Jing, Xiabin.(2008).The immobilization of proteins on biodegradable fibers via biotin-streptavidin bridges.Acta biomaterialia,4(6),1770-1777. |
| MLA | Lu, Tiancheng,et al."The immobilization of proteins on biodegradable fibers via biotin-streptavidin bridges".Acta biomaterialia 4.6(2008):1770-1777. |
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