| Expression and immunohistochemical localization of aquaporin-1 in male reproductive organs of the mouse | |
| Lu, D. Y.3,4; Li, Y.4; Bi, Z. W.4; Yu, H. M.5; Li, X. J.1,2 | |
| 刊名 | Anatomia histologia embryologia
 |
| 2008-02-01 | |
| 卷号 | 37期号:1页码:1-8 |
| ISSN号 | 0340-2096 |
| DOI | 10.1111/j.1439-0264.2007.00827.x |
| 通讯作者 | Li, x. j.(xjli@bjmu.edu.cn) |
| 英文摘要 | Aquaporin-1 (aqp-1), a six-transmembrane domain protein, is found to be responsible for water transport. the purpose of this study was to investigate the expression of aqp-1 mrna and protein in the testis, epididymis, vas deferens, ventral prostate, and seminal vesicle from mature mice by using reverse transcription polymerase chain reaction (rt-pcr) and western blotting, and the cellular localization of aqp-1 by immunohistochemistry. rt-pcr revealed that aqp-1 mrna was expressed in all organs we examined. western blotting displayed a 29-kda band and a 35- to 45-kda band corresponding to non-glycosylated and/or glycosylated aqp-1 in those organs. the immumohistochemical evidence showed that aqp-1 was mainly located on the plasma membrane of epithelial cells of the rete testis, vas deferens, ventral prostate seminal vesicle and the non-ciliated cells of the proximal an distal efferent ducts. however, aqp-1 was absent from spermatogenic cells lining the seminiferous epithelium and from the spermatozoa in the lumen of the distal efferent duct. these findings provide valuable information on the expression of aqp-1 in male reproductive organs and suggest that aqp-1 is involved in water transport to regulate water homeostasis in male reproductive physiology. |
| WOS关键词 | DUCTULI EFFERENTES TESTIS ; MESSENGER-RNA EXPRESSION ; CHIP28 WATER CHANNEL ; HUMAN VAS-DEFERENS ; EPITHELIAL-CELLS ; SPERM MOTILITY ; FLUID REABSORPTION ; MEMBRANE-PROTEIN ; PROXIMAL TUBULE ; GENE-EXPRESSION |
| WOS研究方向 | Anatomy & Morphology ; Veterinary Sciences |
| WOS类目 | Anatomy & Morphology ; Veterinary Sciences |
| 语种 | 英语 |
| 出版者 | WILEY-BLACKWELL |
| WOS记录号 | WOS:000253455200001 |
| 内容类型 | 期刊论文 |
| URI标识 | http://www.corc.org.cn/handle/1471x/2392391 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Li, X. J. |
| 作者单位 | 1.Peking Univ, Hlth Sci Ctr, Dept Pharmacol, Sch Basic Med Sci, Beijing 100083, Peoples R China 2.Peking Univ, Hlth Sci Ctr, State Key Lab Nat & Biomimet Drugs, Beijing 100083, Peoples R China 3.Grad Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Peking Univ, Hlth Sci Ctr, Sch Basic Med Sci, Dept Anat & Histol & Embryol, Beijing 100083, Peoples R China 5.Natl Res Inst Family Planning, Beijing 100081, Peoples R China |
| 推荐引用方式 GB/T 7714 | Lu, D. Y.,Li, Y.,Bi, Z. W.,et al. Expression and immunohistochemical localization of aquaporin-1 in male reproductive organs of the mouse[J]. Anatomia histologia embryologia,2008,37(1):1-8. |
| APA | Lu, D. Y.,Li, Y.,Bi, Z. W.,Yu, H. M.,&Li, X. J..(2008).Expression and immunohistochemical localization of aquaporin-1 in male reproductive organs of the mouse.Anatomia histologia embryologia,37(1),1-8. |
| MLA | Lu, D. Y.,et al."Expression and immunohistochemical localization of aquaporin-1 in male reproductive organs of the mouse".Anatomia histologia embryologia 37.1(2008):1-8. |
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