QQ客服 官方微博 反馈留言

	Generation and characterization of transgenic poplar plants overexpressing a cotton laccase gene
	Wang, Ji1,2; Wang, Chenglong1,2; Zhu, Mulan1,2; Yu, Yang1,2; Zhang, Yuebo3; Wei, Zhiming1
刊名	Plant cell tissue and organ culture
	2008-06-01
卷号	93期号:3页码:303-310
关键词	Laccase Lignin Phenolic metabolism Populus Transgenic poplars
ISSN号	0167-6857
DOI	10.1007/s11240-008-9377-x
通讯作者	Wei, zhiming(zmwei@sippe.ac.cn)
英文摘要	Laccases are copper-containing glycoproteins, which are widespread in higher plants as multigene families. to gain more insight in the function of laccases in plants, especially potential role in lignification, we produced transgenic poplar plants overexpressing a cotton laccase cdna (galac1) under the control of the cauliflower mosaic virus 35s promoter. as compared with untransformed control plants, transgenic plants exhibited a 2.1- to 13.2-fold increased laccase activity, whereas plant growth rate and morphological characters remained similar to control plants. a 2.1-19.6% increase in total lignin content of the stem was found in transgenic plants. moreover, transgenic plants showed a dramatically accelerated oxidation rate of phenolics, without obvious change in total phenolic content. our data suggested that galac1 may participate in lignin synthesis and phenolic metabolism in plants. the present work provided a new genetic evidence for the involvement of plant laccases in lignification.
WOS关键词	POLYPHENOL OXIDASE ; LIGNIN ; ARABIDOPSIS ; RESISTANCE ; ENZYME ; PEROXIDASE ; EXPRESSION ; DEPOSITION ; STRESS ; GENOME
WOS研究方向	Biotechnology & Applied Microbiology ; Plant Sciences
WOS类目	Biotechnology & Applied Microbiology ; Plant Sciences
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000255987000007
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2391353
专题	中国科学院大学
通讯作者	Wei, Zhiming
作者单位	1.Chinese Acad Sci, Shanghai Inst Biol Sci, Shanghai Inst Plant Physiol & Ecol, Shanghai 200032, Peoples R China 2.Chinese Acad Sci, Grad Sch, Shanghai 200032, Peoples R China 3.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Nutrit Sci, Shanghai 200032, Peoples R China
推荐引用方式 GB/T 7714	Wang, Ji,Wang, Chenglong,Zhu, Mulan,et al. Generation and characterization of transgenic poplar plants overexpressing a cotton laccase gene[J]. Plant cell tissue and organ culture,2008,93(3):303-310.
APA	Wang, Ji,Wang, Chenglong,Zhu, Mulan,Yu, Yang,Zhang, Yuebo,&Wei, Zhiming.(2008).Generation and characterization of transgenic poplar plants overexpressing a cotton laccase gene.Plant cell tissue and organ culture,93(3),303-310.
MLA	Wang, Ji,et al."Generation and characterization of transgenic poplar plants overexpressing a cotton laccase gene".Plant cell tissue and organ culture 93.3(2008):303-310.

个性服务

查看访问统计

相关权益政策

暂无数据

收藏/分享

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。