| Electrophysiological characterization of bmk 1, an alpha-like scorpion toxin, on rnav1.5 expressed in hek293t cells | |
| Feng, Xing-Hua2; Chen, Jue-Xu1; Liu, Ying2; Ji, Yong-Hua1 | |
| 刊名 | Toxicology in vitro
 |
| 2008-09-01 | |
| 卷号 | 22期号:6页码:1582-1587 |
| 关键词 | Hek293t cell Cardiac sodium channels Bmk i Patch clamp |
| ISSN号 | 0887-2333 |
| DOI | 10.1016/j.tiv.2008.06.009 |
| 通讯作者 | Ji, yong-hua(yhji@staff.shu.edu.cn) |
| 英文摘要 | A recent study described the pharmacological properties of bmk 1, an alpha-like toxin from the chinese scorpion buthus martensi karsch, on the cardiac sodium channel (hh1) expressed in xenopus oocytes. considering that alpha-like toxins are unique in their inability to bind to rat synaptosomes despite a high toxicity by intravenous injection, the present study investigated the pharmacological properties of bmk i on rnav1.5 expressed in a mammalian hek293t cell line. the results include: (1) bmk i slowed and partially inhibited the inactivation of rnav1.5, produced a substantial persistent current and increased peak current (the ec(50) for increasing peak current by bmk i was 99.4 +/- 20.1 nm); (2) bmk i delayed the recovery of the sodium channel from inactivation; (3) after exposure to 300 nm bmk 1, the steady-state activation curve of rnav1.5 was negatively shifted by about 19 mv; and (4) the association of bmk i and rnav1.5 was faster than their dissociation. the results show that bmk i displayed the pharmacological characteristics of an alpha-like toxin on rnav1.5 channels expressed in hek293t cells, and suggested that the host expression system should be taken into consideration when characterizing the pharmacological properties of toxins. (c) 2008 elsevier ltd. all rights reserved. |
| WOS关键词 | LONG-QT SYNDROME ; CARDIAC SODIUM-CHANNELS ; NA+ CHANNEL ; INACTIVATION GATE ; VENOM ; NEUROTOXINS ; INSECTS ; BINDING ; MODULATION ; MYOCYTES |
| WOS研究方向 | Toxicology |
| WOS类目 | Toxicology |
| 语种 | 英语 |
| 出版者 | PERGAMON-ELSEVIER SCIENCE LTD |
| WOS记录号 | WOS:000259547300023 |
| 内容类型 | 期刊论文 |
| URI标识 | http://www.corc.org.cn/handle/1471x/2391058 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Ji, Yong-Hua |
| 作者单位 | 1.Shanghai Univ, Sch Life Sci, Shanghai 200444, Peoples R China 2.Chinese Acad Sci, Grad Sch, Shanghai Inst Physiol, Inst Biol Sci, Shanghai 200031, Peoples R China |
| 推荐引用方式 GB/T 7714 | Feng, Xing-Hua,Chen, Jue-Xu,Liu, Ying,et al. Electrophysiological characterization of bmk 1, an alpha-like scorpion toxin, on rnav1.5 expressed in hek293t cells[J]. Toxicology in vitro,2008,22(6):1582-1587. |
| APA | Feng, Xing-Hua,Chen, Jue-Xu,Liu, Ying,&Ji, Yong-Hua.(2008).Electrophysiological characterization of bmk 1, an alpha-like scorpion toxin, on rnav1.5 expressed in hek293t cells.Toxicology in vitro,22(6),1582-1587. |
| MLA | Feng, Xing-Hua,et al."Electrophysiological characterization of bmk 1, an alpha-like scorpion toxin, on rnav1.5 expressed in hek293t cells".Toxicology in vitro 22.6(2008):1582-1587. |
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