Characterization of infectious particles of grass carp reovirus by treatment with proteases | |
Fang, Q.1; Seng, E. K.2; Ding, Q. Q.1; Zhang, L. L.1,3 | |
刊名 | Archives of virology
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2008-04-01 | |
卷号 | 153期号:4页码:675-682 |
ISSN号 | 0304-8608 |
DOI | 10.1007/s00705-008-0048-3 |
通讯作者 | Fang, q.(qfang@wh.iov.cn) |
英文摘要 | Proteolytic cleavages play an important role in reovirus infection during entry into cells. the effects of protease digestion on the morphology, infectivity and polypeptide composition of grass carp reovirus (gcrv) were investigated. following treatment with chymotrypsin, the different subviral particles of gcrv were isolated using density gradient centrifugation and examined by electron microscope (em). analysis of protein components revealed that the viral outer capsid was composed of vp5 and vp7. of particular note, vp5 was found to primarily exist within virions as cleaved fragments, which was consistent with observations for its analogue mu 1/mu 1c, generated by autolysis of mu 1 at the mu 1n/mu 1c junction for mammalian orthoreoviruses (mrvs). meanwhile, both trypsin- and chymotrypsin-treated gcrv particles appeared to have an enhanced infectivity. moreover, the corresponding assays between infectivity and protein component indicated that the enhancement of infectivity was correlated to the complete digestion of the outer capsid protein vp7 and partial cleavage of vp5. overall, the results presented in this paper provided strong evidence that the proteins vp5 and vp7 of gcrv play an indispensable role in viral infection. |
WOS关键词 | PROTEOLYTIC ENHANCEMENT ; ROTAVIRUS INFECTIVITY ; MEMBRANE-PENETRATION ; MAMMALIAN REOVIRUSES ; GENUS AQUAREOVIRUS ; SUBVIRAL PARTICLES ; FAMILY REOVIRIDAE ; PROTEIN ; MU-1 ; VIRUS |
WOS研究方向 | Virology |
WOS类目 | Virology |
语种 | 英语 |
出版者 | SPRINGER WIEN |
WOS记录号 | WOS:000254258600008 |
内容类型 | 期刊论文 |
URI标识 | http://www.corc.org.cn/handle/1471x/2390815 |
专题 | 中国科学院大学 |
通讯作者 | Fang, Q. |
作者单位 | 1.Chinese Acad Sci, State Key Lab Virol, Wuhan Inst Viorl, Wuhan 430071, Peoples R China 2.Nanyang Polytech, Sch Chem & Life Sci, Singapore 569830, Singapore 3.Chinese Acad Sci, Grad Sch, Beijing 10039, Peoples R China |
推荐引用方式 GB/T 7714 | Fang, Q.,Seng, E. K.,Ding, Q. Q.,et al. Characterization of infectious particles of grass carp reovirus by treatment with proteases[J]. Archives of virology,2008,153(4):675-682. |
APA | Fang, Q.,Seng, E. K.,Ding, Q. Q.,&Zhang, L. L..(2008).Characterization of infectious particles of grass carp reovirus by treatment with proteases.Archives of virology,153(4),675-682. |
MLA | Fang, Q.,et al."Characterization of infectious particles of grass carp reovirus by treatment with proteases".Archives of virology 153.4(2008):675-682. |
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