Protein separation using free-flow electrophoresis microchip etched in a single step

	Protein separation using free-flow electrophoresis microchip etched in a single step
	Wang, Pingli 1,2; Zhang, Lihua 1; Shan, Yichu 1; Cong, Yongzheng 1,2; Liang, Yu 1,2; Han, Bin 1,3; Liang, Zhen 1; Zhang, Yukui 1
刊名	journal of separation science
	2010-07-01
卷号	33 期号:13 页码:2039-2044
关键词	Fluorescence image Free-flow electrophoresis Microchip One-step etching Protein separation
通讯作者	张丽华
产权排序	1;1
英文摘要	a one-step etching method was developed to fabricate glass free-flow electrophoresis microchips with a rectangle separation microchamber (42 mm-long, 23 mm-wide and 28 mu m-deep), in which two glass bridges (0.5 mm-wide) were made simultaneously to prevent bubbles formed by electrolysis near the pt electrode from entering the separation chamber. by microchip free-flow zone electrophoresis, with 200 v voltage applied, the baseline separation of three fitc labeled proteins, ribonuclease b, myoglobin and beta-lactoglobulin, was achieved, with resolution over 1.78. furthermore, with 2.5 mivl na(2)so(4) added into the electrode buffer to form higher electrical field strength across separation microchamber than electrode compartments, similar resolution of samples was achieved with the applied voltage decreased to 75 v, which could obviously decrease joule heat during continuous separation. all these results demonstrate that the free-flow electrophoresis microchip fabricated by one-step etching method is suitable for the continuous separation of proteins, which might become an effective pre-fractionation method for proteome study.
WOS标题词	science & technology ; physical sciences
类目[WOS]	chemistry, analytical
研究领域[WOS]	chemistry
关键词[WOS]	mass-spectrometry ; device ; chip ; proteomics ; resolution ; speed
收录类别	SCI
原文出处	false
语种	英语
WOS记录号	WOS:000280163900022
公开日期	2010-11-30
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/103567]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China 3.Beijing Inst Technol, Sch Life Sci & Technol, Beijing 100081, Peoples R China
推荐引用方式 GB/T 7714	Wang, Pingli,Zhang, Lihua,Shan, Yichu,et al. Protein separation using free-flow electrophoresis microchip etched in a single step[J]. journal of separation science,2010,33(13):2039-2044.
APA	Wang, Pingli.,Zhang, Lihua.,Shan, Yichu.,Cong, Yongzheng.,Liang, Yu.,...&Zhang, Yukui.(2010).Protein separation using free-flow electrophoresis microchip etched in a single step.journal of separation science,33(13),2039-2044.
MLA	Wang, Pingli,et al."Protein separation using free-flow electrophoresis microchip etched in a single step".journal of separation science 33.13(2010):2039-2044.