Droplet microfluidics for characterizing the neurotoxin-induced responses in individual Caenorhabditis elegans

	Droplet microfluidics for characterizing the neurotoxin-induced responses in individual Caenorhabditis elegans
	Shi, Weiwei; Wen, Hui; Lu, Yao; Shi, Yang; Lin, Bingcheng; Qin, Jianhua
刊名	lab on a chip
	2010
卷号	10 期号:21 页码:2855-2863
通讯作者	林炳承 ; 秦建华
产权排序	1;1
英文摘要	a droplet-based microfluidic device integrated with a novel floatage-based trap array and a tapered immobilization channel array was presented for characterizing the neurotoxin-induced multiple responses in individual caenorhabditis elegans (c. elegans) continuously. the established device enabled the evaluations of movement and fluorescence imaging analysis of individual c. elegans simultaneously. the utility of this device was demonstrated by the pharmacological evaluation of neurotoxin (6-hydroxydopamine, 6-ohda) triggered mobility defects, neuron degeneration and oxidative stress in individual worms. exposure of living worms to 6-ohda could cause obvious mobility defects, selective degeneration of dopaminergic (daergic) neurons, and increased oxidative stress in a dose dependent manner. these results are important towards the understanding of mechanisms leading to daergic toxicity by neurotoxin and will be of benefit for the screening of new therapeutics for neurodegenerative diseases. this device was simple, stable and easy to operate, with the potential to facilitate whole-animal assays and drug screening in a high throughput manner at single animal resolution.
WOS标题词	science & technology ; life sciences & biomedicine ; physical sciences
类目[WOS]	biochemical research methods ; chemistry, multidisciplinary ; nanoscience & nanotechnology
研究领域[WOS]	biochemistry & molecular biology ; chemistry ; science & technology - other topics
关键词[WOS]	c-elegans ; parkinsons-disease ; on-chip ; oxidative stress ; animal-models ; system ; immobilization ; degeneration ; genetics ; neurons
收录类别	SCI
原文出处	false
语种	英语
WOS记录号	WOS:000282808700005
公开日期	2010-11-30
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/103439]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	Chinese Acad Sci, Dalian Inst Chem Phys, Dalian, Peoples R China
推荐引用方式 GB/T 7714	Shi, Weiwei,Wen, Hui,Lu, Yao,et al. Droplet microfluidics for characterizing the neurotoxin-induced responses in individual Caenorhabditis elegans[J]. lab on a chip,2010,10(21):2855-2863.
APA	Shi, Weiwei,Wen, Hui,Lu, Yao,Shi, Yang,Lin, Bingcheng,&Qin, Jianhua.(2010).Droplet microfluidics for characterizing the neurotoxin-induced responses in individual Caenorhabditis elegans.lab on a chip,10(21),2855-2863.
MLA	Shi, Weiwei,et al."Droplet microfluidics for characterizing the neurotoxin-induced responses in individual Caenorhabditis elegans".lab on a chip 10.21(2010):2855-2863.