Sensitive, label-free protein assay using 1-ethyl-3-methylimidazolium tetraflucroborate-supported microchip electrophoresis with laser-induced fluonescence detection | |
Xu, Yuanhong; Li, Jing; Wang, Erkang | |
刊名 | Electrophoresis
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2008-05-01 | |
卷号 | 29期号:9页码:1852-1858 |
关键词 | Ionic liquid Label-free protein assay Microchip Protein |
ISSN号 | 0173-0835 |
DOI | 10.1002/elps.200700499 |
通讯作者 | Wang, erkang(ekwang@ciac.jl.cn) |
英文摘要 | Based on the dimer-monomer equilibrium movement of the fluorescent dye pyronin y (py), a rapid, simple, highly sensitive, label-free method for protein detection was developed by microchip electrophoresis with lif detection. py formed a nonfluorescent dimer induced by the premicellar aggregation of an anionic surfactant, sds, however, the fluorescence intensity of the system increased dramatically when proteins such as bsa, bovine hemoglobin, cytochrome c, and trypsin were added to the solution due to the transition of dimer to fluorescent monomer. furthermore, 1-ethyl-3-methylimidazolium tetrafluoroborate (emimbf(4)) instead of pbs was applied as running buffers in microchip electrophoresis. due to the excellent properties of emimbf4, not only nonspecific protein adsorption was more efficiently suppressed, but also approximately ten-fold higher fluorescence intensity enhancement was obtained than that using pbs. under the optimal conditions, detection limits for bsa, bovine hemoglobin, cytochrome c, and trypsin were 1.00 x 10(-6), 2 x 10(-6), 7 x 10(-7), and 5 x 10(-7) mg/ml, respectively. thus, without covalent modification of the protein, a protein assay method with high sensitivity was achieved on microchips. |
WOS关键词 | INDUCED FLUORESCENCE DETECTION ; TOTAL ANALYSIS SYSTEMS ; POLY(DIMETHYLSILOXANE) MICROFLUIDIC DEVICES ; DODECYL BENZENE SULFONATE ; ACRIDINE-ORANGE DIMER ; BETA-D-MALTOSIDE ; CAPILLARY-ELECTROPHORESIS ; IONIC LIQUIDS ; SURFACE MODIFICATION ; ADSORPTION |
WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
WOS类目 | Biochemical Research Methods ; Chemistry, Analytical |
语种 | 英语 |
出版者 | WILEY-V C H VERLAG GMBH |
WOS记录号 | WOS:000256510000010 |
内容类型 | 期刊论文 |
URI标识 | http://www.corc.org.cn/handle/1471x/2389482 |
专题 | 中国科学院大学 |
通讯作者 | Wang, Erkang |
作者单位 | Chinese Acad Sci, Changchun Inst Appl Chem, Grad Sch, State Key Lab Electroanalyt Chem, Changchun 130022, Jilin, Peoples R China |
推荐引用方式 GB/T 7714 | Xu, Yuanhong,Li, Jing,Wang, Erkang. Sensitive, label-free protein assay using 1-ethyl-3-methylimidazolium tetraflucroborate-supported microchip electrophoresis with laser-induced fluonescence detection[J]. Electrophoresis,2008,29(9):1852-1858. |
APA | Xu, Yuanhong,Li, Jing,&Wang, Erkang.(2008).Sensitive, label-free protein assay using 1-ethyl-3-methylimidazolium tetraflucroborate-supported microchip electrophoresis with laser-induced fluonescence detection.Electrophoresis,29(9),1852-1858. |
MLA | Xu, Yuanhong,et al."Sensitive, label-free protein assay using 1-ethyl-3-methylimidazolium tetraflucroborate-supported microchip electrophoresis with laser-induced fluonescence detection".Electrophoresis 29.9(2008):1852-1858. |
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