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	Sensitive, label-free protein assay using 1-ethyl-3-methylimidazolium tetraflucroborate-supported microchip electrophoresis with laser-induced fluonescence detection
	Xu, Yuanhong; Li, Jing; Wang, Erkang
刊名	Electrophoresis
	2008-05-01
卷号	29期号:9页码:1852-1858
关键词	Ionic liquid Label-free protein assay Microchip Protein
ISSN号	0173-0835
DOI	10.1002/elps.200700499
通讯作者	Wang, erkang(ekwang@ciac.jl.cn)
英文摘要	Based on the dimer-monomer equilibrium movement of the fluorescent dye pyronin y (py), a rapid, simple, highly sensitive, label-free method for protein detection was developed by microchip electrophoresis with lif detection. py formed a nonfluorescent dimer induced by the premicellar aggregation of an anionic surfactant, sds, however, the fluorescence intensity of the system increased dramatically when proteins such as bsa, bovine hemoglobin, cytochrome c, and trypsin were added to the solution due to the transition of dimer to fluorescent monomer. furthermore, 1-ethyl-3-methylimidazolium tetrafluoroborate (emimbf(4)) instead of pbs was applied as running buffers in microchip electrophoresis. due to the excellent properties of emimbf4, not only nonspecific protein adsorption was more efficiently suppressed, but also approximately ten-fold higher fluorescence intensity enhancement was obtained than that using pbs. under the optimal conditions, detection limits for bsa, bovine hemoglobin, cytochrome c, and trypsin were 1.00 x 10(-6), 2 x 10(-6), 7 x 10(-7), and 5 x 10(-7) mg/ml, respectively. thus, without covalent modification of the protein, a protein assay method with high sensitivity was achieved on microchips.
WOS关键词	INDUCED FLUORESCENCE DETECTION ; TOTAL ANALYSIS SYSTEMS ; POLY(DIMETHYLSILOXANE) MICROFLUIDIC DEVICES ; DODECYL BENZENE SULFONATE ; ACRIDINE-ORANGE DIMER ; BETA-D-MALTOSIDE ; CAPILLARY-ELECTROPHORESIS ; IONIC LIQUIDS ; SURFACE MODIFICATION ; ADSORPTION
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
WOS类目	Biochemical Research Methods ; Chemistry, Analytical
语种	英语
出版者	WILEY-V C H VERLAG GMBH
WOS记录号	WOS:000256510000010
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2389482
专题	中国科学院大学
通讯作者	Wang, Erkang
作者单位	Chinese Acad Sci, Changchun Inst Appl Chem, Grad Sch, State Key Lab Electroanalyt Chem, Changchun 130022, Jilin, Peoples R China
推荐引用方式 GB/T 7714	Xu, Yuanhong,Li, Jing,Wang, Erkang. Sensitive, label-free protein assay using 1-ethyl-3-methylimidazolium tetraflucroborate-supported microchip electrophoresis with laser-induced fluonescence detection[J]. Electrophoresis,2008,29(9):1852-1858.
APA	Xu, Yuanhong,Li, Jing,&Wang, Erkang.(2008).Sensitive, label-free protein assay using 1-ethyl-3-methylimidazolium tetraflucroborate-supported microchip electrophoresis with laser-induced fluonescence detection.Electrophoresis,29(9),1852-1858.
MLA	Xu, Yuanhong,et al."Sensitive, label-free protein assay using 1-ethyl-3-methylimidazolium tetraflucroborate-supported microchip electrophoresis with laser-induced fluonescence detection".Electrophoresis 29.9(2008):1852-1858.

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