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	Cloning and characterization of a heat shock protein 70 gene, mshsp70-1, in medicago sativa
	He, Zhishui1,2; Xie, Rong1,2; Wang, Yanzhang1; Zou, Huasong1; Zhu, Jiabi1; Yu, Guanqiao1
刊名	Acta biochimica et biophysica sinica
	2008-03-01
卷号	40期号:3页码:209-216
关键词	Heat shock protein 70 Cdna array Symbiotic nitrogen fixation Nodule development
ISSN号	1672-9145
DOI	10.1111/j.1745-7270.2008.00394.x
通讯作者	Yu, guanqiao(gqyu@sippe.ac.cn)
英文摘要	Some members of the heat shock protein 70 (hsp70) family have important functions in organism development. here, we identified an alfalfa (medicago sativa l. cv. algonquin) hsp70 gene, mshsp70-1, using cdna array and reverse transcription-polymerase chain reaction. this gene contains a 1947 bp open reading frame encoding a protein of 649 amino acids. this protein contains all conserved domains, motifs, and characteristic sequences of plant hsp70s. the expression of mshsp70-1 is enhanced in nodule compared with root, stem, leaf, and flower, and throughout the process of nodule development. northern hybridization analysis indicated that the expression of mshsp70-1 in nodule requires the active baca gene of rhizobia. these results suggested that mshsp70-1 might play an important role in alfalfanodule development.
WOS关键词	HEAT-SHOCK PROTEINS ; MOLECULAR CHAPERONES ; NODULE DEVELOPMENT ; EXPRESSION ; MELILOTI ; ARABIDOPSIS ; TRUNCATULA ; FAMILY ; HSP70
WOS研究方向	Biochemistry & Molecular Biology ; Biophysics
WOS类目	Biochemistry & Molecular Biology ; Biophysics
语种	英语
出版者	BLACKWELL PUBLISHING
WOS记录号	WOS:000254188800004
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2388488
专题	中国科学院大学
通讯作者	Yu, Guanqiao
作者单位	1.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Plant Physiol & Ecol, Natl Lab Plant Mol Genet, Shanghai 200032, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	He, Zhishui,Xie, Rong,Wang, Yanzhang,et al. Cloning and characterization of a heat shock protein 70 gene, mshsp70-1, in medicago sativa[J]. Acta biochimica et biophysica sinica,2008,40(3):209-216.
APA	He, Zhishui,Xie, Rong,Wang, Yanzhang,Zou, Huasong,Zhu, Jiabi,&Yu, Guanqiao.(2008).Cloning and characterization of a heat shock protein 70 gene, mshsp70-1, in medicago sativa.Acta biochimica et biophysica sinica,40(3),209-216.
MLA	He, Zhishui,et al."Cloning and characterization of a heat shock protein 70 gene, mshsp70-1, in medicago sativa".Acta biochimica et biophysica sinica 40.3(2008):209-216.

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