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	Colorimetric oligonucleotide array for genotyping of hepatitis c virus based on the 5 ' non-coding region
	Mao, Hongju1,2; Lu, Zhimeng3; Zhang, Honglian1; Liu, Kangdong1; Zhao, Hanlong1; Jin, Gendi3; Gu, Shimin4; Yang, Mengsu1
刊名	Clinica chimica acta
	2008-02-01
卷号	388期号:1-2页码:22-27
关键词	Hepatitis c virus Genotypes/subtypes Colorimetric oligonucleotide array Dna sequencing
ISSN号	0009-8981
DOI	10.1016/j.cca.2007.09.009
通讯作者	Zhao, hanlong(jlzhao@mail.sim.ac.cn)
英文摘要	Background: hepatitis c virus infection is now one of the most important causes of chronic liver disease. hcv genotype has emerged as an important factor both in predicting a sustained response to, and in determining the duration of, antiviral therapy. microarrays provide excellent tools for rapid genotyping of hepatitis c virus due to their ability to perform a multitude of tests simultaneously. methods: a colorimetric oligonucleotide array that specially and rapidly detects the genotype/subtypes of hepatitis c virus was designed and developed. results: a total of 256 consecutive hcv rna positive samples derived from patients with chronic hepatitis c were examined for their genotypes/subtypes by the oligonucleotide array. identification of genotypes and subtypes by the hcv oligonucleotide array showed a concordance rate of 99.2% with nucleotide sequencing results. conclusion: the oligonucleotide array offers an economical and convenient alternative to detect hcv infection, as well as permitting identification of the genotype/subtypes, in a large-scale setting. oligonucleotide arrays provide a reliable and sensitive method for the detection and discrimination of hcv genotypes common in china, and are suitable for routine use in a diagnostic laboratory. (c) 2007 elsevier b.v all rights reserved.
WOS关键词	FRAGMENT-LENGTH-POLYMORPHISM ; LINE PROBE ASSAY ; HEPATOCELLULAR-CARCINOMA ; PEGINTERFERON ALPHA-2A ; NEISSERIA-GONORRHOEAE ; PHYLOGENETIC ANALYSIS ; SEQUENCE-ANALYSIS ; DNA MICROARRAYS ; INTERFERON ; SUBTYPES
WOS研究方向	Medical Laboratory Technology
WOS类目	Medical Laboratory Technology
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000252770800003
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2388109
专题	中国科学院大学
通讯作者	Zhao, Hanlong
作者单位	1.Chinese Acad Sci, Shanghai Inst Microsyst & Informat Technol, Shanghai 200050, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100864, Peoples R China 3.Shanghai Jiao Tong Univ, Ruijin Hosp, Shanghai, Peoples R China 4.Shanghai Infect Dis Hosp, Shanghai, Peoples R China
推荐引用方式 GB/T 7714	Mao, Hongju,Lu, Zhimeng,Zhang, Honglian,et al. Colorimetric oligonucleotide array for genotyping of hepatitis c virus based on the 5 ' non-coding region[J]. Clinica chimica acta,2008,388(1-2):22-27.
APA	Mao, Hongju.,Lu, Zhimeng.,Zhang, Honglian.,Liu, Kangdong.,Zhao, Hanlong.,...&Yang, Mengsu.(2008).Colorimetric oligonucleotide array for genotyping of hepatitis c virus based on the 5 ' non-coding region.Clinica chimica acta,388(1-2),22-27.
MLA	Mao, Hongju,et al."Colorimetric oligonucleotide array for genotyping of hepatitis c virus based on the 5 ' non-coding region".Clinica chimica acta 388.1-2(2008):22-27.

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