Casein Kinase 2 interacts with human mitogen- and stress-activated protein kinase MSK1 and phosphorylates it at Multiple sites

	Casein Kinase 2 interacts with human mitogen- and stress-activated protein kinase MSK1 and phosphorylates it at Multiple sites
	Shi, Yan 1; Han, Guanghui 2; Wu, Huiling 1; Ye, Kan 1; Tian, Zhipeng 1; Wang, Jiaqi 1; Shi, Huili 1; Ye, Mingliang 2; Zou, Hanfa 2; Huo, Keke 1
刊名	bmb reports
	2009-12-31
卷号	42 期号:12 页码:840-845
关键词	CK2 Mass spectrometry MSK1 Phosphorylation Yeast two-hybrid
通讯作者	叶明亮 ; keke huo
产权排序	2;1
英文摘要	mitogen- and stress-activated protein kinase (msk1) palys; a crucial role in the regulation of transcription downstream of extracellular-signal-regulated kinase1/2 (erk1/2) and mitogen-activated protein kinase p38. msk1 can be phosphorylated and activated in cells by both erk1/2 and p38 alpha. in this study, casein kinase 2 (ck2) was identified as a binding and regulatory partner for msk1. using the yeast two-hybrid system, msk1 was found to interact with the ck2 beta regulatory subunit of ck2. interactions between msk1 and the ck2 alpha catalytic subunit and ck2 beta subunit were demonstrated in vitro and in vivo. we further found that ck2 alpha can only interact with the c-terminal kinase domain of msk1. using site-directed mutagenesis assay and mass spectrometry, we identified five sites in the msk1 c-terminus that could be phosphorylated by ck2 in vitro: ser757, ser758, ser759, ser760 and thr793. of these, ser757, ser759, ser760 and thr793 were previously unknown. [bmb reports 2009; 42(12): 840-845]
WOS标题词	science & technology ; life sciences & biomedicine
类目[WOS]	biochemistry & molecular biology
研究领域[WOS]	biochemistry & molecular biology
关键词[WOS]	creb ; ck2 ; subunits ; mediate ; search
收录类别	SCI
原文出处	false
语种	英语
WOS记录号	WOS:000273334900012
公开日期	2010-11-30
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/103083]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Fudan Univ, Inst Genet, State Key Lab Genet Engn, Sch Life Sci, Shanghai 200433, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China
推荐引用方式 GB/T 7714	Shi, Yan,Han, Guanghui,Wu, Huiling,et al. Casein Kinase 2 interacts with human mitogen- and stress-activated protein kinase MSK1 and phosphorylates it at Multiple sites[J]. bmb reports,2009,42(12):840-845.
APA	Shi, Yan.,Han, Guanghui.,Wu, Huiling.,Ye, Kan.,Tian, Zhipeng.,...&Huo, Keke.(2009).Casein Kinase 2 interacts with human mitogen- and stress-activated protein kinase MSK1 and phosphorylates it at Multiple sites.bmb reports,42(12),840-845.
MLA	Shi, Yan,et al."Casein Kinase 2 interacts with human mitogen- and stress-activated protein kinase MSK1 and phosphorylates it at Multiple sites".bmb reports 42.12(2009):840-845.