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	Integrative proteomic and cytological analysis of the effects of extracellular ca(2+) influx on pinus bungeana pollen tube development
	Wu, Xiaoqin1,2; Chen, Tong1,3; Zheng, Maozhong1,3; Chen, Yanmei1; Teng, Nianjun1,3; Samaj, Jozef4,5; Baluska, Frantisek4,6; Lin, Jinxing1
刊名	Journal of proteome research
	2008-10-01
卷号	7期号:10页码:4299-4312
关键词	Calcium Nifedipine Pinus bungeana Proteomics Pollen tube
ISSN号	1535-3893
DOI	10.1021/pr800241u
通讯作者	Lin, jinxing(linjx@ibcas.ac.cn)
英文摘要	Ca(2+) is an essential ion in the control of pollen germination and tube growth. however, the control of pollen tube development by ca(2+) signaling and its interactions with cytoskeletal components, energy-providing pathways, and cell-expansion machinery remain elusive. here, we used nifedipine (nif) to study ca(2+) functions in differential protein expression and other cellular processes in pinus bungeana pollen tube growth. proteomics analysis indicated that 50 proteins showed differential expression with varying doses of nif. thirty-four of these were homologous to previously reported proteins and were classified into different functional categories closely related to tip-growth machinery. blocking the l-type ca(2+) channel with nif in the pollen tube membrane induced several early alterations within a short time, including a reduction of extracellular ca(2+) influx and a subsequently dramatic decrease in cytosolic free ca(2+) concentration ([ca(2+)]), concomitant with ultrastructural abnormalities and changes in the abundance of proteins involved in energy production and signaling. secondary alterations included actin filament depolymerization, disrupted patterns of endocytosis/exocytosis, and cell wall remodeling, along with changes in the proteins involved in these processes. these results suggested that extracellular ca(2+) ` influx was necessary for the maintenance of the typical tip-focused [ca(2+)] gradient in the p. bungeana pollen tube, and that reduced adenosine triphosphate production (atp), depolymerization of the cytoskeleton, and abnormal endocytosis/exocytosis, together with enhanced rigidity of cell walls, were responsible for the growth arrest observed in pollen tubes treated with nif.
WOS关键词	SELF-INCOMPATIBILITY RESPONSE ; LILY POLLEN ; ACTIN CYTOSKELETON ; LATRUNCULIN-B ; CELL-WALL ; PROTEIN-SYNTHESIS ; PAPAVER-RHOEAS ; CALCIUM INFLUX ; ATP DEPLETION ; TIP GROWTH
WOS研究方向	Biochemistry & Molecular Biology
WOS类目	Biochemical Research Methods
语种	英语
出版者	AMER CHEMICAL SOC
WOS记录号	WOS:000259784300010
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2387814
专题	中国科学院大学
通讯作者	Lin, Jinxing
作者单位	1.Chinese Acad Sci, Key Lab Photosynth & Mol Environm Physiol, Inst Bot, Beijing 100093, Peoples R China 2.Chinese Acad Sci, S China Bot Garden, Guangzhou 510650, Guangdong, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China 4.Univ Bonn, Inst Cellular & Mol Bot, Dept Plant Cell Biol, D-53115 Bonn, Germany 5.Slovak Acad Sci, Inst Plant Genet & Biotechnol, SK-95007 Nitra, Slovakia 6.Slovak Acad Sci, Inst Bot, SK-84223 Bratislava, Slovakia
推荐引用方式 GB/T 7714	Wu, Xiaoqin,Chen, Tong,Zheng, Maozhong,et al. Integrative proteomic and cytological analysis of the effects of extracellular ca(2+) influx on pinus bungeana pollen tube development[J]. Journal of proteome research,2008,7(10):4299-4312.
APA	Wu, Xiaoqin.,Chen, Tong.,Zheng, Maozhong.,Chen, Yanmei.,Teng, Nianjun.,...&Lin, Jinxing.(2008).Integrative proteomic and cytological analysis of the effects of extracellular ca(2+) influx on pinus bungeana pollen tube development.Journal of proteome research,7(10),4299-4312.
MLA	Wu, Xiaoqin,et al."Integrative proteomic and cytological analysis of the effects of extracellular ca(2+) influx on pinus bungeana pollen tube development".Journal of proteome research 7.10(2008):4299-4312.

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