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Overexpressing gsh1 and aspcs1 simultaneously increases the tolerance and accumulation of cadmium and arsenic in arabidopsis thaliana
Guo, Jiangbo1,2; Dai, Xiaojing1,2; Xu, Wenzhong1; Ma, Mi1
刊名Chemosphere
2008-07-01
卷号72期号:7页码:1020-1026
关键词Phytochelatin synthase Glutathione Transgenic Phytoremediation
ISSN号0045-6535
DOI10.1016/j.chemosphere.2008.04.018
通讯作者Ma, mi(mami@ibcas.ac.cn)
英文摘要The goal of this study was to develop transgenic plants with increased tolerance for and accumulation of heavy metals and metalloids from soil by simultaneous overexpression of aspcs1 and gsh1 (derived from garlic and baker's yeast) in arabidopsis thaliana. phytochelatins (pcs) and glutathione (gsh) are the main binding peptides involved in chelating heavy metal ions in plants and other living organisms. single-gene transgenic lines had higher tolerance to and accumulated more cd and as than wild-type. compared to single-gene transgenic lines, dual-gene transformants exhibited significantly higher tolerance to and accumulated more cd and as. one of the dual-gene transgenic lines, pg1, accumulated twice the amount of cd as single-gene transgenic lines. simultaneous overexpression of aspcs1 and gsh1 led to elevated total pc production in transgenic arabidopsis. these results indicate that such a stacking of modified genes is capable of increasing cd and as tolerance and accumulation in transgenic lines, and represents a highly promising new tool for use in phytoremediation efforts. (c) 2008 elsevier ltd. all rights reserved.
WOS关键词GAMMA-GLUTAMYLCYSTEINE SYNTHETASE ; HEAVY-METAL DETOXIFICATION ; PHYTOCHELATIN SYNTHASE ; INDIAN MUSTARD ; TOBACCO PLANTS ; EXPRESSION ; CD ; STRESS ; GENES ; ROLES
WOS研究方向Environmental Sciences & Ecology
WOS类目Environmental Sciences
语种英语
出版者PERGAMON-ELSEVIER SCIENCE LTD
WOS记录号WOS:000258024300004
内容类型期刊论文
URI标识http://www.corc.org.cn/handle/1471x/2386289
专题中国科学院大学
通讯作者Ma, Mi
作者单位1.Chinese Acad Sci, Inst Bot, Key Lab Photosynth & Environm Mol Phys, Beijing 100093, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
推荐引用方式
GB/T 7714
Guo, Jiangbo,Dai, Xiaojing,Xu, Wenzhong,et al. Overexpressing gsh1 and aspcs1 simultaneously increases the tolerance and accumulation of cadmium and arsenic in arabidopsis thaliana[J]. Chemosphere,2008,72(7):1020-1026.
APA Guo, Jiangbo,Dai, Xiaojing,Xu, Wenzhong,&Ma, Mi.(2008).Overexpressing gsh1 and aspcs1 simultaneously increases the tolerance and accumulation of cadmium and arsenic in arabidopsis thaliana.Chemosphere,72(7),1020-1026.
MLA Guo, Jiangbo,et al."Overexpressing gsh1 and aspcs1 simultaneously increases the tolerance and accumulation of cadmium and arsenic in arabidopsis thaliana".Chemosphere 72.7(2008):1020-1026.
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