Human telomeric G-quadruplex formed from duplex under near physiological conditions: Spectroscopic evidence and kinetics

	Human telomeric G-quadruplex formed from duplex under near physiological conditions: Spectroscopic evidence and kinetics
	Zhou, Jun 1,2; Wei, Chunying 1,3; Jia, Guoqing 1,2; Wang, Xiuli 1,2; Feng, Zhaochi 1; Li, Can 1
刊名	biochimie
	2009-09-01
卷号	91 期号:9 页码:1104-1111
关键词	G-quadruplex Duplex Structural interconversion Molecular crowding
通讯作者	李灿
产权排序	1;1
英文摘要	the structural interconversion between the g-quadruplex and duplex in vivo is an important subject. in the present study, we used human telomeric dna duplex composed of ggg(ttaggg)(3)/ccc(taaccc)(3) as a model system to investigate its properties under near physiological conditions by spectroscopic methods. circular dichroism and fluorescence spectra demonstrated that g-quadruplex structure can be formed from duplex at near physiological ph (ph 7.4), salt concentration (150 mm k(+)), and temperature (37 degrees c) in the presence of molecular crowding agent peg (400 g/l), whereas the g-quadruplex structure cannot be formed at 25 degrees c in buffer containing 150 mm k(+) in the presence of peg. it is found that the formation rate of g-quadruplex structure depends on the temperature and the concentrations of both peg and k(+). this work suggests that human telomeric g-quadruplex structure may be potentially formed from watson-crick duplex in vivo. (c) 2009 elsevier masson sas. all rights reserved.
WOS标题词	science & technology ; life sciences & biomedicine
类目[WOS]	biochemistry & molecular biology
研究领域[WOS]	biochemistry & molecular biology
关键词[WOS]	resonance energy-transfer ; g-quartet formation ; molecular crowding conditions ; i-motif structures ; binding-protein ; complementary strand ; dna quadruplexes ; beta-subunit ; equilibrium ; competition
收录类别	SCI
原文出处	false
语种	英语
WOS记录号	WOS:000269654100006
公开日期	2010-11-30
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/102511]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, State Key Lab Catalysis, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China 3.Shanxi Univ, Inst Mol Sci, Minist Educ, Key Lab Chem Biol & Mol Engn, Taiyuan 030006, Peoples R China
推荐引用方式 GB/T 7714	Zhou, Jun,Wei, Chunying,Jia, Guoqing,et al. Human telomeric G-quadruplex formed from duplex under near physiological conditions: Spectroscopic evidence and kinetics[J]. biochimie,2009,91(9):1104-1111.
APA	Zhou, Jun,Wei, Chunying,Jia, Guoqing,Wang, Xiuli,Feng, Zhaochi,&Li, Can.(2009).Human telomeric G-quadruplex formed from duplex under near physiological conditions: Spectroscopic evidence and kinetics.biochimie,91(9),1104-1111.
MLA	Zhou, Jun,et al."Human telomeric G-quadruplex formed from duplex under near physiological conditions: Spectroscopic evidence and kinetics".biochimie 91.9(2009):1104-1111.