| Bjdhns confer heavy-metal tolerance in plants | |
| Xu, Jin1; Zhang, Yu Xiu2; Wei, Wei1; Han, Lu1; Guan, Zi Qiu1; Wang, Zi2; Chai, Tuan Yao1 | |
| 刊名 | Molecular biotechnology
 |
| 2008-02-01 | |
| 卷号 | 38期号:2页码:91-98 |
| 关键词 | Heavy-metal tolerance Brassica juncea l Antisense rna Dehydrin Transgenic tobacco |
| ISSN号 | 1073-6085 |
| DOI | 10.1007/s12033-007-9005-8 |
| 通讯作者 | Chai, tuan yao(tychai@gucas.ac.cn) |
| 英文摘要 | Dehydrin gene transcript could be induced by heavy metals, and some dehydrins possess the ability to bind metals. however, the correlation between dehydrins and heavy-metal stress is unknown. in order to elucidate the contribution of dehydrins to heavy-metal stress tolerance in plants, we cloned two sk2-type dehydrin genes from heavy-metal hyperaccumulator brassica juncea, and investigated their cd/zn tolerance in transgenic plants. semi-quantitative rt-pcr analysis revealed that bjdhn2/bjdhn3 expressed in the leaves, stems and roots at a low level and were up-regulated by heavy metals. antisense bjdhn3 brassica juncea plants showed more electrolyte leakage and higher malondialdehyde production than the control plants when exposed to heavy metals, and the total amount of metals accumulated in the whole plant was reduced. transgenic tobacco plants overexpressing bjdhn2/bjdhn3 showed lower electrolyte leakage and malondialdehyde production than the control plants when exposed to cd/zn. these results indicated that bjdhn2/bjdhn3 enhanced the tolerance for heavy metals by reducing lipid peroxidation and maintaining membrane stability in the plants. |
| WOS关键词 | ARABIDOPSIS-THALIANA ; CITRUS DEHYDRIN ; LOW-TEMPERATURE ; STRESS ; EXPRESSION ; GENES ; LOCALIZATION ; BINDING ; DEHYDRATION ; NUCLEAR |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology |
| WOS类目 | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology |
| 语种 | 英语 |
| 出版者 | HUMANA PRESS INC |
| WOS记录号 | WOS:000252675400001 |
| 内容类型 | 期刊论文 |
| URI标识 | http://www.corc.org.cn/handle/1471x/2384570 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Chai, Tuan Yao |
| 作者单位 | 1.Chinese Acad Sci, Grad Univ, Dept Biol, Beijing 100049, Peoples R China 2.China Univ Min & Technol, Dept Biol Engn, Sch Chem & Environm Engn, Beijing 100083, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xu, Jin,Zhang, Yu Xiu,Wei, Wei,et al. Bjdhns confer heavy-metal tolerance in plants[J]. Molecular biotechnology,2008,38(2):91-98. |
| APA | Xu, Jin.,Zhang, Yu Xiu.,Wei, Wei.,Han, Lu.,Guan, Zi Qiu.,...&Chai, Tuan Yao.(2008).Bjdhns confer heavy-metal tolerance in plants.Molecular biotechnology,38(2),91-98. |
| MLA | Xu, Jin,et al."Bjdhns confer heavy-metal tolerance in plants".Molecular biotechnology 38.2(2008):91-98. |
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