| Brefeldin a disrupts asymmetric spindle positioning in mouse oocytes | |
| Wang, Ling1,2; Wang, Zhen-Bo1,5,6; Zhang, Xuan1; FitzHarris, Greg1; Baltz, Jay M.1,3,4; Sun, Qing-Yuan5; Liu, X. Johne1,2,4 | |
| 刊名 | Developmental biology
 |
| 2008 | |
| 卷号 | 313期号:1页码:155-166 |
| 关键词 | Brefeldin a Oocyte maturation Mouse Polar body Spindle positioning Golgi Midbody Strontium activation Cytokinesis |
| ISSN号 | 0012-1606 |
| DOI | 10.1016/j.ydbio.2007.10.009 |
| 通讯作者 | Liu, x. johne(jliu@ohri.ca) |
| 英文摘要 | Polar body formation in oocytes is an extreme form of asymmetric cell division, but what regulates the asymmetric spindle positioning and cytokinesis is poorly understood. during mouse oocyte maturation, the metaphase i spindle forms at the center but then moves to the cortex prior to anaphase i and first polar body emission. we show here that treating denuded mouse oocytes with brefeldin a, an inhibitor of golgi-based membrane fusion, abolished the asymmetric positioning of the metaphase i spindle and resulted in the formation of two half-size metaphase 11 eggs, instead of a full-sized egg and a polar body. the normal metaphase 11 spindle is similarly asymmetrically positioned in the mature egg, where the spindle lies with its axis parallel to the cortex but becomes perpendicular before anaphase 11 and emission of the second polar body. when ovulated eggs were activated with strontium in the presence of brefeldin a, the metaphase 11 spindle failed to assume perpendicular position, and the chromosomes separated without the extrusion of the second polar body. remarkably, symmetric cytokinesis began following a 3 h delay, forming two half-size eggs each containing a pronucleus. bfa-sensitive intracellular vesicular transport is therefore required for spindle positioning in both mi and mii (c) 2007 elsevier inc. all rights reserved. |
| WOS关键词 | 1ST POLAR BODY ; MEIOTIC SPINDLE ; PROTEINS ; MEIOSIS ; MICROTUBULE ; CYTOKINESIS ; ACTIVATION ; MATURATION ; KINASE ; APPARATUS |
| WOS研究方向 | Developmental Biology |
| WOS类目 | Developmental Biology |
| 语种 | 英语 |
| 出版者 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
| WOS记录号 | WOS:000252300500014 |
| 内容类型 | 期刊论文 |
| URI标识 | http://www.corc.org.cn/handle/1471x/2384246 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Liu, X. Johne |
| 作者单位 | 1.Ottawa Hosp, Ottawa Hlth Res Inst, Ottawa, ON K1Y 4E9, Canada 2.Univ Ottawa, Dept Biochem Microbiol & Immunol, Ottawa, ON, Canada 3.Univ Ottawa, Dept Cellular & Mol Med, Ottawa, ON, Canada 4.Univ Ottawa, Dept Obstet & Gynecol, Ottawa, ON, Canada 5.Chinese Acad Sci, Inst Zool, State Key Lab Reprod Biol, Beijing, Peoples R China 6.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Ling,Wang, Zhen-Bo,Zhang, Xuan,et al. Brefeldin a disrupts asymmetric spindle positioning in mouse oocytes[J]. Developmental biology,2008,313(1):155-166. |
| APA | Wang, Ling.,Wang, Zhen-Bo.,Zhang, Xuan.,FitzHarris, Greg.,Baltz, Jay M..,...&Liu, X. Johne.(2008).Brefeldin a disrupts asymmetric spindle positioning in mouse oocytes.Developmental biology,313(1),155-166. |
| MLA | Wang, Ling,et al."Brefeldin a disrupts asymmetric spindle positioning in mouse oocytes".Developmental biology 313.1(2008):155-166. |
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