Visualization of cd146 dimerization and its regulation in living cells | |
Bu, Pengcheng; Zhuang, Jie; Feng, Jing; Yang, Dongling; Shen, Xun; Yan, Xiyun | |
刊名 | Biochimica et biophysica acta-molecular cell research |
2007-04-01 | |
卷号 | 1773期号:4页码:513-520 |
关键词 | Cd146 Dimerization Fret Nf-kappa b |
ISSN号 | 0167-4889 |
DOI | 10.1016/j.bbamcr.2007.01.009 |
通讯作者 | Yan, xiyun(yanxy@sun5.ibp.ac.cn) |
英文摘要 | Our previous study showed that the adhesion molecule cd146 as a biomarker is over-expressed on activated endothelium during angiogenesis, which was induced by tumor conditional medium and inhibited by anti-cd146 monoclonal antibody (mab aa98). however, the cd146 molecular organization on the cells is unknown. here, using immunoprecipitation, we found that the dimerization of cd146 occurs in both normal and tumor cells. however, the dimer/monomer ratio was higher in tumor cells than in normal cells. moreover, we found that cd146 dimerization was up-regulated by turner conditional medium through the nf-kappa b pathway and down-regulated by mab aa98. to further confirm that cd146 dimerization occurs in living cells, we used fluorescence resonance energy transfer (fret) with melanoma mel888 cells co-expressing cfp/yfp-tagged cd146 fusion proteins. by acceptor photobleaching, we observed a strong fret signal produced by these two fluorescence-tagged proteins. the fret efficiency reached 20.1%. our data provide the first evidence that cd146 dimerization occurs in living cells and is regulated within the tumor microenvironment, implying that dimerization of cd146 may be associated with malignancy. (c) 2007 elsevier b.v. all rights reserved. |
WOS关键词 | ANTI-CD146 MONOCLONAL-ANTIBODY ; ADHESION MOLECULE ; HUMAN-MELANOMA ; TUMOR-GROWTH ; IMMUNOGLOBULIN SUPERFAMILY ; INTERMEDIATE TROPHOBLAST ; INHIBITS ANGIOGENESIS ; ACTIVATION ; EXPRESSION ; PHOSPHORYLATION |
WOS研究方向 | Biochemistry & Molecular Biology ; Cell Biology |
WOS类目 | Biochemistry & Molecular Biology ; Cell Biology |
语种 | 英语 |
出版者 | ELSEVIER SCIENCE BV |
WOS记录号 | WOS:000245784700006 |
内容类型 | 期刊论文 |
URI标识 | http://www.corc.org.cn/handle/1471x/2383722 |
专题 | 中国科学院大学 |
通讯作者 | Yan, Xiyun |
作者单位 | 1.Chinese Acad Sci, Inst Biophys, Natl Lab Biomacromol, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
推荐引用方式 GB/T 7714 | Bu, Pengcheng,Zhuang, Jie,Feng, Jing,et al. Visualization of cd146 dimerization and its regulation in living cells[J]. Biochimica et biophysica acta-molecular cell research,2007,1773(4):513-520. |
APA | Bu, Pengcheng,Zhuang, Jie,Feng, Jing,Yang, Dongling,Shen, Xun,&Yan, Xiyun.(2007).Visualization of cd146 dimerization and its regulation in living cells.Biochimica et biophysica acta-molecular cell research,1773(4),513-520. |
MLA | Bu, Pengcheng,et al."Visualization of cd146 dimerization and its regulation in living cells".Biochimica et biophysica acta-molecular cell research 1773.4(2007):513-520. |
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