Metabonomics study of liver cancer based on ultra performance liquid chromatography coupled to mass spectrometry with HILIC and RPLC separations

	Metabonomics study of liver cancer based on ultra performance liquid chromatography coupled to mass spectrometry with HILIC and RPLC separations
	Chen, Jing 1; Wang, Wenzhao 1; Lv, Shen 2; Yin, Peiyuan 1; Zhao, Xinjie 1; Lu, Xin 1; Zhang, Fengxia 1; Xu, Guowang 1
刊名	analytica chimica acta
	2009-09-14
卷号	650 期号:1 页码:3-9
关键词	Metabonomics Metabolomics Hydrophilic interaction chromatography Ultra performance liquid chromatography-mass spectrometry Liver cancer Biomarker
通讯作者	许国旺
产权排序	1;1
英文摘要	in this study, urinary metabolites from liver cancer patients and healthy volunteers were studied by a metabonomic method based on ultra performance liquid chromatography coupled to mass spectrometry. both hydrophilic interaction chromatography (hilic) and reversed-phase liquid chromatography (rplc) were used to separate the urinary metabolites. principle component analysis (pca) and partial least squares to latent structure-discriminant analysis (pls-da) models were built to separate the healthy volunteers from the liver cancer patients and to find compounds that are expressed in significantly different amounts between the two populations. 21 metabolite ions were considered as potential biomarkers according to the variable importance in the project (vip) value and s-plot. compared with rplc, a more sensitive and stable response can be recorded in hilic mode due to the high content of organic solvent used. moreover, the liver cancer group and the healthy volunteers can be better separated based on the data from the hilic separation, which indicates that hilic is suitable for urinary metabonomic analysis. in hilic mode, several polar compounds related to arginine and proline metabolism, alanine and aspartate metabolism, lysine degradation, nicotinate and nicotinamide metabolism were found to be significantly changed in the concentrations of the two different populations: healthy and cancer. in contrast, in rplc mode, these changed compounds are related to fatty acids oxidation. (c) 2009 elsevier b.v. all rights reserved.
WOS标题词	science & technology ; physical sciences
类目[WOS]	chemistry, analytical
研究领域[WOS]	chemistry
关键词[WOS]	hydrophilic interaction chromatography ; prolidase deficiency ; rat urine ; tof-ms ; identification ; metabolomics ; electrospray ; discovery ; column
收录类别	SCI
原文出处	false
语种	英语
WOS记录号	WOS:000270003800002
公开日期	2010-11-30
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/102171]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China 2.Dalian Med Univ, Affiliated Hosp 2, Dalian 116023, Peoples R China
推荐引用方式 GB/T 7714	Chen, Jing,Wang, Wenzhao,Lv, Shen,et al. Metabonomics study of liver cancer based on ultra performance liquid chromatography coupled to mass spectrometry with HILIC and RPLC separations[J]. analytica chimica acta,2009,650(1):3-9.
APA	Chen, Jing.,Wang, Wenzhao.,Lv, Shen.,Yin, Peiyuan.,Zhao, Xinjie.,...&Xu, Guowang.(2009).Metabonomics study of liver cancer based on ultra performance liquid chromatography coupled to mass spectrometry with HILIC and RPLC separations.analytica chimica acta,650(1),3-9.
MLA	Chen, Jing,et al."Metabonomics study of liver cancer based on ultra performance liquid chromatography coupled to mass spectrometry with HILIC and RPLC separations".analytica chimica acta 650.1(2009):3-9.