QQ客服 官方微博 反馈留言

	Characterization of a novel plant promoter specifically induced by heavy metal and identification of the promoter regions conferring heavy metal responsiveness
	Qi, Xiaoting; Zhang, Yuxiu; Chai, Tuanyao
刊名	Plant physiology
	2007
卷号	143期号:1页码:50-59
ISSN号	0032-0889
DOI	10.1104/pp.106.080283
通讯作者	Chai, tuanyao(tychai@gucas.ac.cn)
英文摘要	The bean (phaseolus vulgaris) stress-related gene number 2 (pvsr2) gene responds to heavy metals but not to other forms of environmental stresses. to elucidate its heavy metal-regulatory mechanism at the transcriptional level, we isolated and characterized the promoter region (-1623/+48) of pvsr2. deletions from the 5' end revealed that a sequence between -222 and -147 relative to the transcriptional start site was sufficient for heavy metal-specific induction of the promoter region of pvsr2. detailed analysis of this 76-bp fragment indicated that heavy metal-responsive elements were localized in two regions (-222/-188 and -187/-147), each of which could separately confer heavy metal-responsive expression on the beta-glucuronidase gene in the context of a minimal cauliflower mosaic virus 35s promoter. region i (-222/-188) contains a motif (metal-regulatory element-like sequence) similar to the consensus metal-regulatory element of the animal metallothionein gene, and mutation of this motif eliminated the heavy metal-inducible function of region i. region ii (-187/-147) had no similarity to previously identified cis-acting elements involved in heavy metal induction, suggesting the presence of a novel heavy metal-responsive element. transformed tobacco (nicotiana tabacum) seedlings expressing beta-glucuronidase under control of the pvsr2 promoter region (-687/+48) showed heavy metal-specific responsive activity that depended on the type and concentration of the heavy metal and the type of organ. these findings further our understanding of the regulation of pvsr2 expression and provide a new heavy-metal-inducible promoter system in transgenic plants.
WOS关键词	METALLOTHIONEIN GENE ; RICE METALLOTHIONEIN ; SUPEROXIDE-DISMUTASE ; REGULATORY ELEMENTS ; RESPONSE ELEMENT ; FERRITIN GENE ; COPPER ; EXPRESSION ; CADMIUM ; SEQUENCE
WOS研究方向	Plant Sciences
WOS类目	Plant Sciences
语种	英语
出版者	AMER SOC PLANT BIOLOGISTS
WOS记录号	WOS:000243350600008
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2382957
专题	中国科学院大学
通讯作者	Chai, Tuanyao
作者单位	1.Chinese Acad Sci, Grad Univ, Dept Biol, Beijing 100049, Peoples R China 2.China Univ Min & Technol, Dept Bioengn, Sch Chem & Environm Engn, Beijing 100083, Peoples R China
推荐引用方式 GB/T 7714	Qi, Xiaoting,Zhang, Yuxiu,Chai, Tuanyao. Characterization of a novel plant promoter specifically induced by heavy metal and identification of the promoter regions conferring heavy metal responsiveness[J]. Plant physiology,2007,143(1):50-59.
APA	Qi, Xiaoting,Zhang, Yuxiu,&Chai, Tuanyao.(2007).Characterization of a novel plant promoter specifically induced by heavy metal and identification of the promoter regions conferring heavy metal responsiveness.Plant physiology,143(1),50-59.
MLA	Qi, Xiaoting,et al."Characterization of a novel plant promoter specifically induced by heavy metal and identification of the promoter regions conferring heavy metal responsiveness".Plant physiology 143.1(2007):50-59.

个性服务

查看访问统计

相关权益政策

暂无数据

收藏/分享

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。