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	Transient expression of osteopontin in hek 293 cells in serum-free culture
	Han, Xiangzong; Sun, Lei; Fang, Qiangyi; Li, Dongxiao; Gong, Xianghui; Wu, Yuyin; Yang, Shengli; Shen, Bing Q.
刊名	Enzyme and microbial technology
	2007-07-02
卷号	41期号:1-2页码:133-140
关键词	Hek 293 Osteopontin Polyethylenimine Purification Transient expression
ISSN号	0141-0229
DOI	10.1016/j.enzmictec.2006.12.013
通讯作者	Shen, bing q.(bqshen@srcb.ac.cn)
英文摘要	As a highly phosphorylated glycoprotein, opn has important implications in the pathogenesis of many types of cancers and autoimmune diseases. with the green fluorescent protein (gfp) as a reporter, the parameters to transfect hek 293-f cells were optimized. these included the concentrations of plasmid dna and linear 25 kda pei, the reaction time for dna/pei complex formation and the time to transfect the cells. when the cloned full-length human osteopontin (opn-a) gene was transiently expressed, a limited growth of cells and excessive accumulations of lactate and ammonium were observed in shaker flask culture. however, when compared to 27 mu g/ml in shaker flask culture, the yield of opn-a reached 49 mu g/ml in a 5 l bioreactor as a result of the doubled maximal viable cell density in the presence of 1 g/l protein hydrolysates in the post-transfection growth medium. the purified opn-a was proven to induce the cell adhesion after chromatographic purification. (c) 2006 elsevier inc. all rights reserved.
WOS关键词	BONE SIALOPROTEIN ; PROTEIN-PRODUCTION ; MAMMALIAN-CELLS ; GENE-EXPRESSION ; LUNG-CANCER ; TRANSFECTION ; SUSPENSION ; ARTHRITIS ; INTEGRIN ; BINDING
WOS研究方向	Biotechnology & Applied Microbiology
WOS类目	Biotechnology & Applied Microbiology
语种	英语
出版者	ELSEVIER SCIENCE INC
WOS记录号	WOS:000247107700020
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2382708
专题	中国科学院大学
通讯作者	Shen, Bing Q.
作者单位	1.Chinese Acad Sci, Grad Sch, Shanghai 200233, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Biol Sci, Shanghai 200233, Peoples R China 3.UCB Pharma, Cambridge, MA 02139 USA
推荐引用方式 GB/T 7714	Han, Xiangzong,Sun, Lei,Fang, Qiangyi,et al. Transient expression of osteopontin in hek 293 cells in serum-free culture[J]. Enzyme and microbial technology,2007,41(1-2):133-140.
APA	Han, Xiangzong.,Sun, Lei.,Fang, Qiangyi.,Li, Dongxiao.,Gong, Xianghui.,...&Shen, Bing Q..(2007).Transient expression of osteopontin in hek 293 cells in serum-free culture.Enzyme and microbial technology,41(1-2),133-140.
MLA	Han, Xiangzong,et al."Transient expression of osteopontin in hek 293 cells in serum-free culture".Enzyme and microbial technology 41.1-2(2007):133-140.

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