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	Osrrm, a spen-like rice gene expressed specifically in the endosperm
	Chen, Shi-Yan; Wang, Zong-Yang; Cai, Xiu-Ling
刊名	Cell research
	2007-08-01
卷号	17期号:8页码:713-721
关键词	Rrm Spen Promoter trap Gus Rice
ISSN号	1001-0602
DOI	10.1038/cr.2007.43
通讯作者	Cai, xiu-ling(xlcai@sippe.ac.cn)
英文摘要	We used the promoter trap technique to identify a rice plant, named 107(#), in which the beta-glucuronidase (gus) reporter gene was expressed specifically in the endosperm. a single copy of the t-dna was inserted into the plant genome, and a candidate gene osrrm was identified by the insertion. the osrrm promoter directed gus expression specifically in rice endosperm, analogous to the gus expression pattern observed in 107(#). osrrm is a single-copy gene in rice and encodes a nuclear protein containing 1005 amino-acid residues with two rna recognition motifs and one spen paralog and ortholog c-terminal domain. western blot analysis confirmed that the osrrm protein was specifically expressed in rice endosperm. ectopic expression of osrrm in transgenic plants led to abnormalities, such as short stature, retarded growth and low fructification rates. our data, in conjunction with the reported function of spen genes, implicated osrrm in the regulation of cell development in rice endosperm.
WOS关键词	PROTEINS ; ARABIDOPSIS ; MOTIF ; ENCODES ; BINDING ; CLONING ; MAIZE ; TOOLS
WOS研究方向	Cell Biology
WOS类目	Cell Biology
语种	英语
出版者	INST BIOCHEMISTRY & CELL BIOLOGY
WOS记录号	WOS:000249774500007
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2382620
专题	中国科学院大学
通讯作者	Cai, Xiu-Ling
作者单位	1.Chinese Acad Sci, Shanghai Inst Biol Sci, Natl Key Lab Plant Mol Genet, Shanghai 200032, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	Chen, Shi-Yan,Wang, Zong-Yang,Cai, Xiu-Ling. Osrrm, a spen-like rice gene expressed specifically in the endosperm[J]. Cell research,2007,17(8):713-721.
APA	Chen, Shi-Yan,Wang, Zong-Yang,&Cai, Xiu-Ling.(2007).Osrrm, a spen-like rice gene expressed specifically in the endosperm.Cell research,17(8),713-721.
MLA	Chen, Shi-Yan,et al."Osrrm, a spen-like rice gene expressed specifically in the endosperm".Cell research 17.8(2007):713-721.

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