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	The isolation of 1,2,3,4,6-penta-o-galloyl-beta-d-glucose from acer truncatum bunge by high-speed counter-current chromatography
	Zhao, Wen-Hua; Gao, Chun-Chun; Ma, Xiao-Feng; Bai, Xiao-Yu; Zhang, Ying-Xia
刊名	Journal of chromatography b-analytical technologies in the biomedical and life sciences
	2007-05-01
卷号	850期号:1-2页码:523-527
关键词	1,2,3,4,6-penta-o-galloyl-beta-d-glucose Acer truncatum bunge Counter-current chromatography
ISSN号	1570-0232
DOI	10.1016/j.jchromb.2006.11.002
通讯作者	Zhang, ying-xia(zwhua@ccmu.edu.cn)
英文摘要	High-speed counter-current chromatography (hsccc) was successfully used for the isolation and purification of 1,2,3,4,6-penta-o-galloyl-beta-d-glucose from the ethyl acetate extract of the leaves of acer truncatum bunge using a two-phase system composed of n-hexane-ethyl acetate-methanol-water at a volume ratio of (0.25:5:1:5, v/v/v/v) for the first time. each injection of 80 mg crude extract yielded 7.25 mg of pure 1,2,3,4,6-penta-o-galloyl-beta-d-glucose. high-performance liquid chromatography (hplc) analyses of the ccc fraction revealed that the purity of 1,2,3,4,6-penta-o-galloyl-beta-d-glucose was over 95%. (c) 2006 elsevier b.v. all rights reserved.
WOS关键词	PREPARATIVE ISOLATION ; PURIFICATION ; SEPARATION ; EXTRACT ; CELLS ; ISOFLAVONES ; POLYPHENOLS ; INHIBITION ; INDUCTION ; ALKALOIDS
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
WOS类目	Biochemical Research Methods ; Chemistry, Analytical
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000246703500067
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2382385
专题	中国科学院大学
通讯作者	Zhang, Ying-Xia
作者单位	1.Capital Med Univ, Dept Biol Chem, Beijing 100069, Peoples R China 2.Harbin Commercial Univ, Dept Pharm, Harbin 150076, Peoples R China 3.Grad Univ, Chinese Acad Sci, Dept Biol, Beijing 100049, Peoples R China 4.Capital Med Univ, Lab Liver Protect & Regulat, Beijing 100069, Peoples R China
推荐引用方式 GB/T 7714	Zhao, Wen-Hua,Gao, Chun-Chun,Ma, Xiao-Feng,et al. The isolation of 1,2,3,4,6-penta-o-galloyl-beta-d-glucose from acer truncatum bunge by high-speed counter-current chromatography[J]. Journal of chromatography b-analytical technologies in the biomedical and life sciences,2007,850(1-2):523-527.
APA	Zhao, Wen-Hua,Gao, Chun-Chun,Ma, Xiao-Feng,Bai, Xiao-Yu,&Zhang, Ying-Xia.(2007).The isolation of 1,2,3,4,6-penta-o-galloyl-beta-d-glucose from acer truncatum bunge by high-speed counter-current chromatography.Journal of chromatography b-analytical technologies in the biomedical and life sciences,850(1-2),523-527.
MLA	Zhao, Wen-Hua,et al."The isolation of 1,2,3,4,6-penta-o-galloyl-beta-d-glucose from acer truncatum bunge by high-speed counter-current chromatography".Journal of chromatography b-analytical technologies in the biomedical and life sciences 850.1-2(2007):523-527.

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