| Muc1 c-terminal heterodimer and its tumorigenicity | |
| Ling, Wei; Yi Yan-Ping; Xuan, Liu; Kufe, Donald; Cheng, Cao | |
| 刊名 | Progress in biochemistry and biophysics
 |
| 2007-04-01 | |
| 卷号 | 34期号:4页码:375-381 |
| 关键词 | Muc1 C-ter Cqc motif Heterodimer |
| ISSN号 | 1000-3282 |
| 通讯作者 | Cheng, cao(caoc@nic.bmi.ac.cn) |
| 英文摘要 | The muc1 protein is expressed as a stable heterodimer from a single polypeptide, which was cleaved into two subunits in endoplasmic reticulum. it localizes at the cell membrane as an alpha/beta-complex, tethered by the beta-subunit transmembrane domain. previous studies implicated that the three amino acids of the transmembrane domain adjacent to the cytoplasmic domain in muc1 beta-subunit are the residues cys-gln-cys (cqc). therein, site-directed mutagenesis of the cqc motif was performed and the cell lines were established. these cell lines include hct116/muc1, hct116/muc1 (cqc -> aqa), hct116/muc1 (delta cqcrrk), hct116/muc1c-ter (cqc -> aqa), which can express wild type or mutant muc1 on the cell surface, or its cytoplasmic domain. the effects of cqc -> aqa mutation or cqcrrk deletion were investigated in vitro and in vivo. compared with wild type muc1, the mutants depressed soft agar colony formation and showed abrogated tumorigenicity in nude mice. these findings implicate that cqcrrk motif mediate the formation of wc i protein complex. as a result of this research, disruption of muc1-c-terminal subunit-associated dimerization by mutation of cqc -> aqa might represent a novel therapeutic approach for tumor. |
| WOS关键词 | CYTOPLASMIC DOMAIN ; BETA-CATENIN ; CARCINOMA ; PROTEIN ; GENE ; PHOSPHORYLATION ; RECEPTOR ; ANTIGEN ; KINASE ; CELLS |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biophysics |
| WOS类目 | Biochemistry & Molecular Biology ; Biophysics |
| 语种 | 英语 |
| 出版者 | SCIENCE CHINA PRESS |
| WOS记录号 | WOS:000245908200007 |
| 内容类型 | 期刊论文 |
| URI标识 | http://www.corc.org.cn/handle/1471x/2381173 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Cheng, Cao |
| 作者单位 | 1.Acad Mil Med Sci, Beijing Inst Biotechnol, Beijing 100850, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China 3.Harvard Univ, Sch Med, Dana Farber Canc Inst, Boston, MA 02115 USA |
| 推荐引用方式 GB/T 7714 | Ling, Wei,Yi Yan-Ping,Xuan, Liu,et al. Muc1 c-terminal heterodimer and its tumorigenicity[J]. Progress in biochemistry and biophysics,2007,34(4):375-381. |
| APA | Ling, Wei,Yi Yan-Ping,Xuan, Liu,Kufe, Donald,&Cheng, Cao.(2007).Muc1 c-terminal heterodimer and its tumorigenicity.Progress in biochemistry and biophysics,34(4),375-381. |
| MLA | Ling, Wei,et al."Muc1 c-terminal heterodimer and its tumorigenicity".Progress in biochemistry and biophysics 34.4(2007):375-381. |
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