Improved protein separation by microchip isoelectric focusing with stepwise gradient of electric field strength

	Improved protein separation by microchip isoelectric focusing with stepwise gradient of electric field strength
	Cong, Yongzheng 1,2; Liang, Yu 1; Zhang, Lihua 1; Zhang, Weibing 1; Zhang, Yukui 1
刊名	journal of separation science
	2009-02-01
卷号	32 期号:3 页码:462-465
关键词	IEF Microchip Protein separation Stepwise gradient of electric field strength
通讯作者	张丽华
产权排序	1;1
英文摘要	a stepwise gradient of electric field strength was proposed for microchip ief-based protein separation, by which after focusing at low voltages, ief was performed by applying higher separation voltages step-by-step. a linear relationship between the focusing time and the inverse of the electric field strength was found. in addition, the conductivity of an established ph gradient showed a negative but nonlinear correlation with the applied voltage. based on the above-mentioned results, a stepwise gradient of electric field strength, ranging from 160 to 1500 v/cm was applied in the separation of proteins extracted from escherichia coli in a straight glass microchip channel permanently coated by polyacrylamide. compared to the conventional separation performed under a constant field strength of 750 v/cm, the increased stepwise gradient of electric field strength resulted in improved resolution and decreased focusing time, while without the negative effects of joule heat for protein separation. all these results demonstrated that such a method might be of great significance to achieve high resolution and high-throughput analysis of complex protein samples for microchip ief.
WOS标题词	science & technology ; physical sciences
类目[WOS]	chemistry, analytical
研究领域[WOS]	chemistry
关键词[WOS]	plastic microfluidic devices ; electrophoresis ; chip ; microchannels ; adaptation
收录类别	SCI
原文出处	false
语种	英语
WOS记录号	WOS:000263385600018
公开日期	2010-11-30
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/101679]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Cong, Yongzheng,Liang, Yu,Zhang, Lihua,et al. Improved protein separation by microchip isoelectric focusing with stepwise gradient of electric field strength[J]. journal of separation science,2009,32(3):462-465.
APA	Cong, Yongzheng,Liang, Yu,Zhang, Lihua,Zhang, Weibing,&Zhang, Yukui.(2009).Improved protein separation by microchip isoelectric focusing with stepwise gradient of electric field strength.journal of separation science,32(3),462-465.
MLA	Cong, Yongzheng,et al."Improved protein separation by microchip isoelectric focusing with stepwise gradient of electric field strength".journal of separation science 32.3(2009):462-465.