Formaldehyde at low concentration induces protein tau into globular amyloid-like aggregates in vitro and in vivo | |
Nie, Chun Lai1; Wei, Yan1; Chen, Xinyong2; Liu, Yan Ying1; Dui, Wen1; Liu, Ying1; Davies, Martyn C.2; Tendler, Saul J. B.2; He, Rong Giao1 | |
刊名 | Plos one
![]() |
2007-07-18 | |
卷号 | 2期号:7页码:13 |
ISSN号 | 1932-6203 |
DOI | 10.1371/journal.pone.0000629 |
通讯作者 | He, rong giao(herq@sun5.ibp.ac.cn) |
英文摘要 | Recent studies have shown that neurodegeneration is closely related to misfolding and aggregation of neuronal tau. our previous results show that neuronal tau aggregates in formaldehyde solution and that aggregated tau induces apoptosis of sh-sy5y and hippocampal cells. in the present study, based on atomic force microscopy (afm) observation, we have found that formaldehyde at low concentrations induces tau polymerization whilst acetaldehyde does not. neuronal tau misfolds and aggregates into globular-like polymers in 0.01-0.1% formaldehyde solutions. apart from globular-like aggregation, no fibril-like polymerization was observed when the protein was incubated with formaldehyde for 15 days. sds-page results also exhibit tau polymerizing in the presence of formaldehyde. under the same experimental conditions, polymerization of bovine serum albumin (bsa) or alpha-synuclein was not markedly detected. kinetic study shows that tau significantly misfolds and polymerizes in 60 minutes in 0.1% formaldehyde solution. however, presence of 10% methanol prevents protein tau from polymerization. this suggests that formaldehyde polymerization is involved in tau aggregation. such aggregation process is probably linked to the tau's special "worm-like'' structure, which leaves the epsilon-amino groups of lys and thiol groups of cys exposed to the exterior. such a structure can easily bond to formaldehyde molecules in vitro and in vivo. polymerizing of formaldehyde itself results in aggregation of protein tau. immunocytochemistry and thioflavin s staining of both endogenous and exogenous tau in the presence of formaldehyde at low concentrations in the cell culture have shown that formaldehyde can induce tau into amyloid-like aggregates in vivo during apoptosis. the significant protein tau aggregation induced by formaldehyde and the severe toxicity of the aggregated tau to neural cells may suggest that toxicity of methanol and formaldehyde ingestion is related to tau misfolding and aggregation. |
WOS研究方向 | Science & Technology - Other Topics |
WOS类目 | Multidisciplinary Sciences |
语种 | 英语 |
出版者 | PUBLIC LIBRARY SCIENCE |
WOS记录号 | WOS:000207452100018 |
内容类型 | 期刊论文 |
URI标识 | http://www.corc.org.cn/handle/1471x/2380744 |
专题 | 中国科学院大学 |
通讯作者 | He, Rong Giao |
作者单位 | 1.Chinese Acad Sci, Inst Biophys, Grad Sch, State Key Lab Brain & Cognit Sci, Beijing 100080, Peoples R China 2.Univ Nottingham, Sch Pharm, Lab Biophys & Surface Anal, Nottingham NG7 2RD, England |
推荐引用方式 GB/T 7714 | Nie, Chun Lai,Wei, Yan,Chen, Xinyong,et al. Formaldehyde at low concentration induces protein tau into globular amyloid-like aggregates in vitro and in vivo[J]. Plos one,2007,2(7):13. |
APA | Nie, Chun Lai.,Wei, Yan.,Chen, Xinyong.,Liu, Yan Ying.,Dui, Wen.,...&He, Rong Giao.(2007).Formaldehyde at low concentration induces protein tau into globular amyloid-like aggregates in vitro and in vivo.Plos one,2(7),13. |
MLA | Nie, Chun Lai,et al."Formaldehyde at low concentration induces protein tau into globular amyloid-like aggregates in vitro and in vivo".Plos one 2.7(2007):13. |
个性服务 |
查看访问统计 |
相关权益政策 |
暂无数据 |
收藏/分享 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。
修改评论