Synthesis and characterization of a new boronate affinity monolithic capillary for specific capture of cis-diol-containing compounds

	Synthesis and characterization of a new boronate affinity monolithic capillary for specific capture of cis-diol-containing compounds
	Ren, Lianbing 1; Liu, Zhen 1; Dong, Mingming 1; Ye, Mingliang 2; Zou, Hanfa 2
刊名	journal of chromatography a
	2009-06-05
卷号	1216 期号:23 页码:4768-4774
关键词	Boronate affinity Capillary HPLC cis-Diol Enrichment Monolithic column
ISSN号	0021-9673
通讯作者	邹汉法
产权排序	2;4
英文摘要	boronate affinity chromatography (bac) is an important tool for specific capture and separation of cis-diol-containing compounds such as glycoproteins, rna and carbohydrates. only a few reports on monolithic column-based bac have appeared. in this paper, boronate functionalized monolithic capillary column was synthesized by in situ free radical polymerization for the first time. the prepared column was first characterized in terms of morphology, pore properties, capacity and retention mechanisms. the column exhibited uniform open channel network and high capture capacity. systematical investigation on the retention mechanism revealed that multiple intermolecular interactions occur between the analytes and the boronate affinity monolith, including boronate affinity, reversed-phase, cation-exchange and hydrogen bonding interactions, depending on the conditions used. in addition. the presence of lewis base such as fluoride ion in the mobile phase was found to be favorable to the complexation between cis-diol-containing compounds with the boronic acid ligand under less basic conditions. on the basis of these fundamental investigations, the prepared monolithic column was then applied to the capture of adenosine and flavin adenine dinucleotide. the investigations in this study provide sound understanding not only on how to manipulate the separation selectivity through selection of appropriate mobile phase composition on the currently prepared columns but also on how to design next-generation columns with desired properties and functions. (c) 2009 elsevier b.v. all rights reserved.
WOS标题词	science & technology ; life sciences & biomedicine ; physical sciences
类目[WOS]	biochemical research methods ; chemistry, analytical
研究领域[WOS]	biochemistry & molecular biology ; chemistry
关键词[WOS]	shotgun proteome analysis ; performance liquid-chromatography ; maldi-tof/tof-ms ; stationary phases ; reversed-phase ; esi-ms/ms ; separation ; columns ; proteins ; electrochromatography
收录类别	SCI
原文出处	true
语种	英语
WOS记录号	WOS:000266449800009
公开日期	2010-11-30
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/101645]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Nanjing Univ, Key Lab Analyt Chem Life Sci, Sch Chem & Chem Engn, Nanjing 210093, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China
推荐引用方式 GB/T 7714	Ren, Lianbing,Liu, Zhen,Dong, Mingming,et al. Synthesis and characterization of a new boronate affinity monolithic capillary for specific capture of cis-diol-containing compounds[J]. journal of chromatography a,2009,1216(23):4768-4774.
APA	Ren, Lianbing,Liu, Zhen,Dong, Mingming,Ye, Mingliang,&Zou, Hanfa.(2009).Synthesis and characterization of a new boronate affinity monolithic capillary for specific capture of cis-diol-containing compounds.journal of chromatography a,1216(23),4768-4774.
MLA	Ren, Lianbing,et al."Synthesis and characterization of a new boronate affinity monolithic capillary for specific capture of cis-diol-containing compounds".journal of chromatography a 1216.23(2009):4768-4774.