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	Identification and characterization of sanh and sani involved in the hydroxylation of pyridyl residue during nikkomycin biosynthesis in streptomyces ansochromogenes
	Xie, Zhoujie; Niu, Guoqing; Li, Rui; Liu, Gang; Tan, Huarong
刊名	Current microbiology
	2007-12-01
卷号	55期号:6页码:537-542
ISSN号	0343-8651
DOI	10.1007/s00284-007-9028-1
通讯作者	Tan, huarong(tanhr@sun.im.ac.cn)
英文摘要	Nikkomycins are highly potent inhibitors of chitin synthase. the nikkomycin biosynthetic gene cluster has been cloned from streptomyces asochromogenes. two cytochrome p450 monooxygenase genes (sanq, sanh) and one ferredoxin gene (sani) were found in the cluster. it was reported that sanq is involved in the hydroxylation of l-his, a key step in 4-formyl-4-imidazolin-2-one base biosynthesis. here, we have studied the function of sanh and sani. disruption of sanh abolished the production of nikkomycin x and z, but it accumulated one dominant component nikkomycin lx, which is the nikkomycin x analog lacking the hydroxy group at the pyridyl residue. the sani disruption mutant accumulated predominantly nikkomycin lx in addition to nikkomycin x and z. the nikkomycin production profile of the sanh and sani double disruption mutant was the same as that of the sanh disruption mutant. these results confirmed that sanh is essential for the hydroxylation of pyridyl residue in nikkomycin biosynthesis of s. ansochromogenes and first demonstrated that sani is an effective electron donor for sanh, but not for sanq in vivo.
WOS关键词	NUCLEOSIDE ANTIBIOTIC NIKKOMYCIN ; TENDAE TU901 ; MOLECULAR CHARACTERIZATION ; CYTOCHROME-P450 ; GENE ; CLONING ; MOIETY
WOS研究方向	Microbiology
WOS类目	Microbiology
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000250407000014
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2380525
专题	中国科学院大学
通讯作者	Tan, Huarong
作者单位	1.Chinese Acad Sci, Inst Microbiol, State Key Lab Microbial Resources, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China 3.Univ Oklahoma, Hlth Sci Ctr, Coll Dent, Oklahoma City, OK 73104 USA
推荐引用方式 GB/T 7714	Xie, Zhoujie,Niu, Guoqing,Li, Rui,et al. Identification and characterization of sanh and sani involved in the hydroxylation of pyridyl residue during nikkomycin biosynthesis in streptomyces ansochromogenes[J]. Current microbiology,2007,55(6):537-542.
APA	Xie, Zhoujie,Niu, Guoqing,Li, Rui,Liu, Gang,&Tan, Huarong.(2007).Identification and characterization of sanh and sani involved in the hydroxylation of pyridyl residue during nikkomycin biosynthesis in streptomyces ansochromogenes.Current microbiology,55(6),537-542.
MLA	Xie, Zhoujie,et al."Identification and characterization of sanh and sani involved in the hydroxylation of pyridyl residue during nikkomycin biosynthesis in streptomyces ansochromogenes".Current microbiology 55.6(2007):537-542.

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