Chitosan oligosaccharides attenuate hydrogen peroxide-induced stress injury in human umbilical vein endothelial cells

	Chitosan oligosaccharides attenuate hydrogen peroxide-induced stress injury in human umbilical vein endothelial cells
	Liu, Hong-Tao 1,2,3; Li, Wen-Ming 1; Xu, Gang 1; Li, Xiu-Ying 1; Bai, Xue-Fang 2; Wei, Peng 2; Yu, Chao 1; Du, Yu-Guang 2
刊名	pharmacological research
	2009-03-01
卷号	59 期号:3 页码:167-175
关键词	Chitosan oligosaccharides Human umbilical vein endothelial cells Hydrogen peroxide Apoptosis
ISSN号	issn:1043-6618
通讯作者	杜昱光
产权排序	1;1
英文摘要	chitosan oligosaccharides (cos) have been reported to have anticancer activity, immuno-enhancing effect and antimicrobial activity. however, other biological activities are unknown. herein, we investigated the protective effects of cos against hydrogen peroxide (h2o2)-induced oxidative damage on human umbilical vein endothelial cells (huvec, ecv304 cells). after 24 h pre-incubation with cos (25-200 mu g/ml), the viability loss in ecv304 cells induced by h2o2(300 mu m) for 12 h was markedly restored in a concentration-dependent manner as measured by mtt assay. this effect was accompanied by a marked decrease in intracellular reactive oxygen species (ros) by measuring intensity of dcfh fluorescence. cos also exerted preventive effects on suppressing the production of lipid pet-oxidation such as malondialdehyde (mda), restoring activities of endogenous antioxidants including superoxide dismutase (sod) and glutathione peroxidase (gsh-px), along with the capacity of increasing levels of nitric oxide (no) and nitric oxide synthase (nos), as were determined by commercial regent kits. in addition, pre-incubation of cos with ecv304 cells for 24 h resulted in the reduction of apoptosis and the induction of cell cycle arrest in g(1)/s + m phase as assayed quantitatively by annexin v-fluorescein isothiocyanate (fitc) apoptosis detection kit using flow cytometry. taken together, our findings suggest that cos can effectively protect huvecs against oxidative stress by h2o2, which might be of importance in the treatment of cardiovascular diseases. (c) 2008 elsevier ltd. all rights reserved.
WOS标题词	science & technology ; life sciences & biomedicine
类目[WOS]	pharmacology & pharmacy
研究领域[WOS]	pharmacology & pharmacy
关键词[WOS]	nitric-oxide synthase ; activated protein-kinase ; kappa-b activation ; in-vitro ; oxidative stress ; pc12 cells ; h2o2-induced apoptosis ; antioxidant activities ; shear-stress ; chitooligosaccharides
收录类别	SCI
原文出处	true
语种	英语
WOS记录号	WOS:000264615100004
公开日期	2010-11-30
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/101557]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Chongqing Med Univ, Inst Basic Med Sci, Chongqing 400016, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing 110864, Peoples R China
推荐引用方式 GB/T 7714	Liu, Hong-Tao,Li, Wen-Ming,Xu, Gang,et al. Chitosan oligosaccharides attenuate hydrogen peroxide-induced stress injury in human umbilical vein endothelial cells[J]. pharmacological research,2009,59(3):167-175.
APA	Liu, Hong-Tao.,Li, Wen-Ming.,Xu, Gang.,Li, Xiu-Ying.,Bai, Xue-Fang.,...&Du, Yu-Guang.(2009).Chitosan oligosaccharides attenuate hydrogen peroxide-induced stress injury in human umbilical vein endothelial cells.pharmacological research,59(3),167-175.
MLA	Liu, Hong-Tao,et al."Chitosan oligosaccharides attenuate hydrogen peroxide-induced stress injury in human umbilical vein endothelial cells".pharmacological research 59.3(2009):167-175.