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	Development of a heat shock inducible and inheritable rnai system in silkworm
	Dai, Hongjiu1,2,4; Jiang, Rongjing1,2; Wang, Jue2; Xu, Guojiang2; Cao, Meixun1; Wang, Zhugang2; Fei, Jian2,3
刊名	Biomolecular engineering
	2007-12-01
卷号	24期号:6页码:625-630
关键词	Transgenic silkworm Rnai Heat shock Eclosion hormone Bombyx mori
ISSN号	1389-0344
DOI	10.1016/j.bioeng.2007.10.004
通讯作者	Jiang, rongjing(rjjiang@sibs.ac.cn)
英文摘要	A heat shock inducible and inheritable rna interference (rnai) system was developed in the silkworm (bombyx mori). rnai transgenic silkworms were generated by injecting silkworm eggs with a piggybac transposon plasmid carrying rnai sequence against target gene driven by the drosophila heat shock protein 70 (hsp70) promoter and the helper plasmid expressing piggybac transposase. the transgenic egfp gene and the endogenous eclosion hormone (eh) gene were chosen respectively as the target genes. in the rnai transgenic, silkworms, heat shock at 42 degrees c significantly and specifically reduced the expression of egfp or eh gene in silkworms according to the corresponding rnai targeting sequence but not in silkworms with the irrelevant rnai sequence demonstrating the efficiency and specificity of the rnai effect. heat shock in the pupal stage hampered pupal-adult eclosion and reduced egg fertility in eh rnai transgenic silkworms but not in the wild type or egfp rnai transgenic silkworms. the establishment of this heat inducible and inheritable conditional rna interference system in silkworms provided an approach for the first time to dissect the functions of target genes in silkworms at different stages. (c) 2007 elsevier b.v. all rights reserved.
WOS关键词	DOUBLE-STRANDED-RNA ; MANDUCA-SEXTA ; ECLOSION HORMONE ; TOBACCO HORNWORM ; BOMBYX-MORI ; GENE ; EXPRESSION ; SEQUENCE ; ECDYSIS ; INTERFERENCE
WOS研究方向	Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Genetics & Heredity
WOS类目	Biochemical Research Methods ; Biotechnology & Applied Microbiology ; Genetics & Heredity
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000252063800007
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2380248
专题	中国科学院大学
通讯作者	Jiang, Rongjing
作者单位	1.Chinese Acad Sci, SIBS, Inst Plant Physiol & Ecol, Shanghai 200032, Peoples R China 2.Shanghai Nan Fang Res Ctr Model Organism, Shanghai 201203, Peoples R China 3.Tongji Univ, Shanghai 200092, Peoples R China 4.Chinese Acad Sci, Grad Sch, Beijing 100864, Peoples R China
推荐引用方式 GB/T 7714	Dai, Hongjiu,Jiang, Rongjing,Wang, Jue,et al. Development of a heat shock inducible and inheritable rnai system in silkworm[J]. Biomolecular engineering,2007,24(6):625-630.
APA	Dai, Hongjiu.,Jiang, Rongjing.,Wang, Jue.,Xu, Guojiang.,Cao, Meixun.,...&Fei, Jian.(2007).Development of a heat shock inducible and inheritable rnai system in silkworm.Biomolecular engineering,24(6),625-630.
MLA	Dai, Hongjiu,et al."Development of a heat shock inducible and inheritable rnai system in silkworm".Biomolecular engineering 24.6(2007):625-630.

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