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	Rna interference-mediated silencing of phytochelatin synthase gene reduce cadmium accumulation in rice seeds
	Li, Jiang-Chuan; Guo, Jiang-Bo; Xu, Wen-Zhong; Ma, Mi
刊名	Journal of integrative plant biology
	2007-07-01
卷号	49期号:7页码:1032-1037
关键词	Accumulation Cadmium Phytochelatin synthase Rice Rna interference
ISSN号	1672-9072
DOI	10.1111/j.1672-9072.2007.00473.x
通讯作者	Ma, mi(mami@ibcas.ac.cn)
英文摘要	Phytochelatins (pcs) play an important role in heavy metal resistance and accumulation. to reduce the accumulation of cadmium (cd) in rice seeds, the expression of phytochelatin synthase (pcs) gene ospcs1 was suppressed by rna interference (rnai). a hairpin construct of a pcs fragment was designed in the prnai-ospcs1 under the control of zmm1, a seed-specific promoter from maize. the construct was introduced into rice (japonica) through agrobacterium tumefaciens. the rnai rice plantlets were selected and cultivated in pots exposured to 10 mg/kg cd. the transcriptional level of ospcs1 declined in seeds of some rnai rice compared to the wild type. as a result cd accumulation was reduced by about half in the seeds of rnai rice. as expected, no apparent difference of growth appeared between rnai and wild-type plants. the results suggest that this new approach can be used to control heavy metal accumulation in crops.
WOS关键词	HEAVY-METAL DETOXIFICATION ; ARABIDOPSIS-THALIANA ; BINDING PEPTIDES ; PLANTS ; TOLERANCE ; METALLOTHIONEINS ; BIOSYNTHESIS ; ROLES
WOS研究方向	Biochemistry & Molecular Biology ; Plant Sciences
WOS类目	Biochemistry & Molecular Biology ; Plant Sciences
语种	英语
出版者	WILEY-BLACKWELL
WOS记录号	WOS:000247442100008
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2380180
专题	中国科学院大学
通讯作者	Ma, Mi
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Photosynth & Environm Mol Physiol, Beijing 100093, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	Li, Jiang-Chuan,Guo, Jiang-Bo,Xu, Wen-Zhong,et al. Rna interference-mediated silencing of phytochelatin synthase gene reduce cadmium accumulation in rice seeds[J]. Journal of integrative plant biology,2007,49(7):1032-1037.
APA	Li, Jiang-Chuan,Guo, Jiang-Bo,Xu, Wen-Zhong,&Ma, Mi.(2007).Rna interference-mediated silencing of phytochelatin synthase gene reduce cadmium accumulation in rice seeds.Journal of integrative plant biology,49(7),1032-1037.
MLA	Li, Jiang-Chuan,et al."Rna interference-mediated silencing of phytochelatin synthase gene reduce cadmium accumulation in rice seeds".Journal of integrative plant biology 49.7(2007):1032-1037.

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