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	Cathepsin d is secreted from m-be cells: its potential role as a biomarker of lung cancer
	Lou, Xiaomin; Xiao, Ting; Zhao, Kang; Wang, Hao; Zheng, Hongwei; Lin, Dongmei; Lu, Youyong; Gao, Yanning; Cheng, Shujun; Liu, Siqi
刊名	Journal of proteome research
	2007-03-01
卷号	6期号:3页码:1083-1092
关键词	Lung cancer Conditioned media Secretory protein Cathepsin d
ISSN号	1535-3893
DOI	10.1021/pr060422t
通讯作者	Xu, ningzhi(xunzh@genomics.org.cn)
英文摘要	The early diagnosis of lung cancer is an effective approach to reduce the mortality caused by malignancy. to explore serum biomarkers of lung cancer at early stage, m-be, a sv40t-transformed human bronchial epithelial cell line with the phenotypic features of early tumorigenesis at high passage, was cultured in the conditioned media to collect its secretory proteins. the proteins secreted from different passage m-be cells were extracted and then separated by two-dimensional electrophoresis (2-de). maldi-tof/tof mass spectrometry was adopted to identify the passage-dependent 2-de spots. totally, 47 proteins were identified, including 23 that were up-regulated and 24 that were down-regulated. of these proteins, cathepsin d was a typical secretory protein that exhibited the increased abundance either in culture media or in cells during passaging. furthermore, the proteomic conclusions were validated in the clinical samples of lung cancer patients. when sandwich elisa was used, the concentrations of cathepsin d in plasma showed significant differences between lung squamous cell carcinomas (scc, 104 cases) and normal donors (36 cases, p <= 0.015). when tissue microarray (tma) was used, cathepsin d expression levels in scc tissues (178 cases) were significantly higher than those in normal donors (40 cases, p < 0.001). the present study has revealed that m-be cells at different passages could secrete or release some proteins into the living environment, which might serve as the potential resource for exploring the biomarkers of lung cancer.
WOS关键词	BREAST-CANCER ; PROCATHEPSIN-D ; D EXPRESSION ; PROTEIN ; CARCINOMA ; SERUM ; MANNOSE-6-PHOSPHATE ; PHOSPHORYLATION ; ADENOCARCINOMA ; FIBROBLASTS
WOS研究方向	Biochemistry & Molecular Biology
WOS类目	Biochemical Research Methods
语种	英语
出版者	AMER CHEMICAL SOC
WOS记录号	WOS:000244638400016
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2380089
专题	中国科学院大学
通讯作者	Xu, Ningzhi
作者单位	1.Chinese Acad Sci, Div Proteom, Genom Inst, Beijing 101318, Peoples R China 2.Chinese Acad Med Sci, Dept Etiol & Carcinogenesis, Canc Inst & Hosp, Beijing 100021, Peoples R China 3.Peking Union Med Coll, Beijing 100021, Peoples R China 4.Peking Univ, Sch Oncol, Inst Canc Res, Beijing 100034, Peoples R China 5.Chinese Acad Med Sci, Lab Cell & Mol Biol, Canc Inst & Canc Hosp, Beijing 100021, Peoples R China 6.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Lou, Xiaomin,Xiao, Ting,Zhao, Kang,et al. Cathepsin d is secreted from m-be cells: its potential role as a biomarker of lung cancer[J]. Journal of proteome research,2007,6(3):1083-1092.
APA	Lou, Xiaomin.,Xiao, Ting.,Zhao, Kang.,Wang, Hao.,Zheng, Hongwei.,...&Xu, Ningzhi.(2007).Cathepsin d is secreted from m-be cells: its potential role as a biomarker of lung cancer.Journal of proteome research,6(3),1083-1092.
MLA	Lou, Xiaomin,et al."Cathepsin d is secreted from m-be cells: its potential role as a biomarker of lung cancer".Journal of proteome research 6.3(2007):1083-1092.

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