QQ客服 官方微博 反馈留言

	Characterization and expression of beta-1,3-glucanase genes in jujube fruit induced by the microbial biocontrol agent cryptococcus laurentii
	Tian, Shi P.; Yao, Hong J.; Deng, Xin; Xu, Xiang B.; Qin, Guo Z.; Chan, Zhu L.
刊名	Phytopathology
	2007-03-01
卷号	97期号:3页码:260-268
ISSN号	0031-949X
DOI	10.1094/phyto-97-3-0260
通讯作者	Tian, shi p.(tsp@ibcas.ac.cn)
英文摘要	Two beta-1,3-glucanase genes were cloned from jujube (ziziphus jujuba mill) fruit and designated glu-1 and glu-2 (genbank accession numbers dq012940 and dq093571), respectively. the expression of glu-1 and glu-2 in jujube fruit in response to wounding and microbial biocontrol agent was evaluated by semi-quantitative reverse-transcription polymerase chain reaction analysis. wounding and treatment with cryptococcus laurentii stimulated an increase in beta-1,3-glucanase (ec 3.2.1.39) activity in jujube fruit. analysis of gene expression proved that glu-1 was highly induced both by wounding and c. laurentii, whereas glu-2 was broadly not responsive to the yeast. the expression of glu-1 was noticeably enhanced with increased concentrations of c. laurentii, suggesting that glu-1 may play a role in defense responses to fungal pathogens. the results hold true at the levels of gene activation and enzyme accumulation in jujube fruit treated by both stimuli, resulting in a significant decrease in disease incidence and lesion diameter, thus providing evidence that changes in beta-1,3-glucanase activity are related to expression of the genes. taken together, these findings suggest the possible use of beta-1,3-glucanase activity as a biochemical marker for screening jujube fruit against fungal pathogens, and also provide a mechanistic framework for the functions of beta-1,3-glucanase in defense responses.
WOS关键词	SYSTEMIC ACQUIRED-RESISTANCE ; POSTHARVEST BIOLOGICAL-CONTROL ; PATHOGENESIS-RELATED PROTEINS ; BOTRYTIS-CINEREA ; DISEASE RESISTANCE ; TOMATO SEEDS ; APPLE FRUIT ; PHYTOPHTHORA-INFESTANS ; ANTAGONISTIC YEASTS ; DEFENSE RESPONSES
WOS研究方向	Plant Sciences
WOS类目	Plant Sciences
语种	英语
出版者	AMER PHYTOPATHOLOGICAL SOC
WOS记录号	WOS:000244613400001
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2380047
专题	中国科学院大学
通讯作者	Tian, Shi P.
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Photosynth & Mol Environm Physiol, Beijing 100093, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Tian, Shi P.,Yao, Hong J.,Deng, Xin,et al. Characterization and expression of beta-1,3-glucanase genes in jujube fruit induced by the microbial biocontrol agent cryptococcus laurentii[J]. Phytopathology,2007,97(3):260-268.
APA	Tian, Shi P.,Yao, Hong J.,Deng, Xin,Xu, Xiang B.,Qin, Guo Z.,&Chan, Zhu L..(2007).Characterization and expression of beta-1,3-glucanase genes in jujube fruit induced by the microbial biocontrol agent cryptococcus laurentii.Phytopathology,97(3),260-268.
MLA	Tian, Shi P.,et al."Characterization and expression of beta-1,3-glucanase genes in jujube fruit induced by the microbial biocontrol agent cryptococcus laurentii".Phytopathology 97.3(2007):260-268.

个性服务

查看访问统计

相关权益政策

暂无数据

收藏/分享

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。