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	Involvement of sphingoid bases in mediating reactive oxygen intermediate production and programmed cell death in arabidopsis
	Shi, Lihua1,2; Bielawski, Jacek3; Mu, Jinye1,2; Dong, Haili1,2; Teng, Chong1,2; Zhang, Jian1; Yang, Xiaohui1; Tomishige, Nario4; Hanada, Kentaro4; Hannun, Yusuf A.3
刊名	Cell research
	2007-12-01
卷号	17期号:12页码:1030-1040
关键词	Arabidopsis Fumonisin b1 resistant11-1 Pcd Rois Sphingolipids
ISSN号	1001-0602
DOI	10.1038/cr.2007.100
通讯作者	Zuo, jianru(jrzuo@genetics.ac.cn)
英文摘要	Sphingolipids have been suggested to act as second messengers for an array of cellular signaling activities in plant cells, including stress responses and programmed cell death (pcd). however, the mechanisms underpinning these processes are not well understood. here, we report that an arabidopsis mutant, fumonisin b resistant11-1 (fbr11-1), which fails to generate reactive oxygen inter-mediates (rois), is incapable of initiating pcd when the mutant is challenged by famonisin b-1(fb1), a specific inhibitor of ceramide synthase. molecular analysis indicated that fbr11 encodes a long-chain base 1 (lcb1) subunit of serine palmitoyltransferase (spt), which catalyzes the first rate-limiting step of de novo sphingolipid synthesis. mass spectrometric analysis of the sphingolipid concentrations revealed that whereas the fbr11-1 mutation did not affect basal levels of sphingoid bases, the mutant showed attenuated formation of sphingoid bases in response to fb1. by a direct feeding experiment, we show that the free sphingoid bases dilrydrosphingosine, phytosphingosine and sphingosine efficiently induce roi generation followed by cell death. conversely, roi generation and cell death induced by dilrydrosphingosine were specifically blocked by its phosphorylated form dihydrosphingosine-1-phosphate in a dose-dependent manner, suggesting that the maintenance of homeostasis between a free sphingoid base and its phosphorylated derivative is critical to determining the cell fate. because alterations of the sphingolipid level occur prior to the roi production, we propose that the free sphingoid bases are involved in the control of pcd in arabidopsis, presumably through the regulation of the roi level upon receiving different developmental or environmental cues.
WOS关键词	PLANT-DISEASE RESISTANCE ; F-SP LYCOPERSICI ; SERINE PALMITOYLTRANSFERASE ; HYPERSENSITIVE RESPONSE ; AAL-TOXIN ; SIGNALING PATHWAYS ; MAMMALIAN-CELLS ; LCB1 SUBUNIT ; GENE ; SPHINGOLIPIDS
WOS研究方向	Cell Biology
WOS类目	Cell Biology
语种	英语
出版者	INST BIOCHEMISTRY & CELL BIOLOGY
WOS记录号	WOS:000252607800009
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2380006
专题	中国科学院大学
通讯作者	Zuo, Jianru
作者单位	1.Chinese Acad Sci, Inst Genet & Dev Biol, Natl Key Lab Plant Genom, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China 3.Med Univ S Carolina, Dept Biochem & Mol Biol, Charleston, SC 29425 USA 4.Natl Inst Infect Dis, Dept Biochem & Cell Biol, Shinjuku Ku, Tokyo 1628640, Japan
推荐引用方式 GB/T 7714	Shi, Lihua,Bielawski, Jacek,Mu, Jinye,et al. Involvement of sphingoid bases in mediating reactive oxygen intermediate production and programmed cell death in arabidopsis[J]. Cell research,2007,17(12):1030-1040.
APA	Shi, Lihua.,Bielawski, Jacek.,Mu, Jinye.,Dong, Haili.,Teng, Chong.,...&Zuo, Jianru.(2007).Involvement of sphingoid bases in mediating reactive oxygen intermediate production and programmed cell death in arabidopsis.Cell research,17(12),1030-1040.
MLA	Shi, Lihua,et al."Involvement of sphingoid bases in mediating reactive oxygen intermediate production and programmed cell death in arabidopsis".Cell research 17.12(2007):1030-1040.

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